Detailed information of TA system TA161809

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2386709..2387347
• Replicon: chromosome
• Accession: NZ_CP055181
• Organism: Shigella flexneri strain STEFF_17

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: HUZ45_RS11580
• Protein ID: WP_000813794.1
• Coordinates: 2387171..2387347 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HUZ45_RS11575
• Protein ID: WP_001270286.1
• Coordinates: 2386709..2387125 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ45_RS11555 (2381861)	2381861..2382802	-	942	WP_152914928.1	ABC transporter permease	-
HUZ45_RS11560 (2382803)	2382803..2383816	-	1014	WP_000220393.1	ABC transporter ATP-binding protein	-
HUZ45_RS11565 (2383834)	2383834..2384979	-	1146	WP_000047418.1	ABC transporter substrate-binding protein	-
HUZ45_RS11570 (2385224)	2385224..2386630	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
HUZ45_RS11575 (2386709)	2386709..2387125	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
HUZ45_RS11580 (2387171)	2387171..2387347	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
HUZ45_RS11585 (2387569)	2387569..2387799	+	231	WP_000494244.1	YncJ family protein	-
HUZ45_RS11590 (2387891)	2387891..2389852	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
HUZ45_RS11595 (2389925)	2389925..2390461	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
HUZ45_RS11600 (2390553)	2390553..2391728	+	1176	WP_001236265.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T161809 WP_000813794.1 NZ_CP055181:c2387347-2387171 [Shigella flexneri]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T161809 NZ_CP071319:c1254391-1253999 [Acinetobacter indicus]
ATGTCCACGGCTAAGCGCGACTTTCAGCTGAAACCGAGCCGTCTGGCGCTGCTGTTTCAGCTGAGCCTGCTGATTCTATT
TTCTGTTTTAAGTTTTCAATTATTACATCCGCTGCTTTGGCTGCTAGGTCTGCTCCTGGCGGCTGTCAGTTATTACGGGT
TTCAGCGACGCTCCCAATTAACCGCCTTGGCGCATTTAGATGGGGCAGAGTGGAGTCTGCAGTTTAATTCTGGCTCCAAG
ATTCAACGCGGTCTGCTCGAGCATATTCTGGATCATCAGCTGTATCTGGTGCTGTATTTTCATCATGCACCGGTCAAATC
TGCAGTCATCTGGCGTGACCAAGTTTCGTTTGCAGAGTGGAAAGCCTTAAAAACATTGGCAAAAACCATCTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT161809 WP_001270286.1 NZ_CP055181:c2387125-2386709 [Shigella flexneri]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT161809 NZ_CP071319:c1254635-1254378 [Acinetobacter indicus]
ATGAGTGAAGACATTAGCTTAGAAGAGCGTAAAGTGATTTATCGTGCCCGTCGCGGTTTAAAGGAAATTGATGTCTACTT
TGATCCTTATGTGAAAAACTATTATTTAACCGCTGATCCGGAAGAAAAAGCCACGTTTGCGGCGCTGGTTGAACAGGAAG
ATCCGGATTTACTGGACTGGTTTATGGAAGTGTCTGAACCGCCACGCCCGGAAATGCGCGAGCTGATCCGTAAATTAAAA
CACTATGTCCACGGCTAA

Structures

Toxin

• PDB: 6HPB