Detailed information of TA system TA161808

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2294864..2296452
• Replicon: chromosome
• Accession: NZ_CP055181
• Organism: Shigella flexneri strain STEFF_17

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: S1ELM3
• Locus tag: HUZ45_RS11170
• Protein ID: WP_001125439.1
• Coordinates: 2295130..2296452 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: HUZ45_RS11165
• Protein ID: WP_001301023.1
• Coordinates: 2294864..2295130 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ45_RS11165 (2294864)	2294864..2295130	+	267	WP_001301023.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
HUZ45_RS11170 (2295130)	2295130..2296452	+	1323	WP_001125439.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
HUZ45_RS11175 (2296739)	2296739..2297686	-	948	WP_096178406.1	hypothetical protein	-
HUZ45_RS11180 (2298501)	2298501..2298860	-	360	WP_096178407.1	hypothetical protein	-
HUZ45_RS11185 (2299189)	2299189..2299929	-	741	WP_239638181.1	hypothetical protein	-
HUZ45_RS11190 (2300324)	2300324..2300532	+	209	Protein_2197	AraC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49275.82 Da
• Isoelectric Point: 8.2482

>T161808 WP_001125439.1 NZ_CP055181:2295130-2296452 [Shigella flexneri]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVESNVLRLHGRLSREYGSK

Nucleotide

• Length: 1323 bp

>T161808 NZ_CP071319:234824-234988 [Acinetobacter indicus]
ATGTCTAGAGGTGATAAGTCGGCCTATACCGACAAGCAGAAACGTCAGGCCCGCCATATTATTGAATCGGAAAAAGAGCG
AGGCCATTCCGAAGAAGAAGCAGAACGCATTGGCTGGTCTACCGTCAATAAACAGGATGGCGGCGGTAAAAAGAAATCCC
ACTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10016.38 Da
• Isoelectric Point: 6.2267

>AT161808 WP_001301023.1 NZ_CP055181:2294864-2295130 [Shigella flexneri]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDAKNASPES
TEQQNLEW

Nucleotide

• Length: 267 bp

>AT161808 NZ_CP071319:233873-234742 [Acinetobacter indicus]
ATGAATCATTATCCTGACAGCCCATCTCCGCAAGAGAAACAGGAGCATCAGCCCGGTGTGCAATATAAAATGACGCCCGA
ACCTGAAATTATTAAAAGCAATTATCAGGGCAGTCAAAAACTCAAAGACAAGGTGGCCTTAATTACCGGCGGTGATAGCG
GCATCGGCCGCTCCGTGGCAGTGCTGTTTGCCCGCGAAGGTGCCAATATTATTATTCCCTATTTAGAAGAAGATCAGGAT
GCGCAAGACACCAAGCGACTGGTTGAGCAGGAAGGTCGTCAATGTCTGCTGATTCAGTGTGACCTGCGCCAACATGCGGC
AGTGCAGCAGATGGTCGATCTGGCTTTGCAGCAGTTTCCGCATATTAATATTCTGGTCAATAATGCTGCTGTGCAATATC
CACAAAAAACCATTGAAGATATTAATCCCGAACAGCTGCATAAAACCTTTGAAACCAACCTCTACCCGCTGTTTTATGTG
ACCCAGCTGCTGATTCCTCAAATGCAGCCCGGTGACAGTATTATCAATACCACCAGTATCACCAGTTATCAGGGCCATAA
TCAGCTGATTGATTATGCCAGTACCAAAGGGGCAATTACTGCTTTTACCCGTAGCCTGTCGACCAACTTGCTGAAAAATA
AAACCGGGATTCGGGTGAATGGTGTCGCACCCGGCCCAATCTGGACGCCGTTAATTCCAAGCAGTTTTGATGAAGAACAG
CTAAAAGGTTTTGGTGAAGATACCCCCATGGGCCGCATGGGTCAGCCAAGTGAAGTTGCGCCGGCTTATCTGTTTTTAGC
CTCCGATGAAGCCAGCTATATTTCCGGTCAGGTGATTCATGTCAATGGCGGTACCATTGTCAATGGTTAA

Structures

Toxin

• PDB: 5K98
• PDB: 3TPV
• PDB: 3DNV
• PDB: 3TPD
• PDB: 3HZI
• PDB: 3FBR
• PDB: 4YG7
• PDB: 3TPE
• PDB: 2WIU
• PDB: 3DNT
• PDB: 3TPB
• PDB: 3TPT
• PDB: 3DNU