Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1044195..1044778 Replicon chromosome
Accession NZ_CP055181
Organism Shigella flexneri strain STEFF_17

Toxin (Protein)


Gene name mazF Uniprot ID S1EZP4
Locus tag HUZ45_RS05130 Protein ID WP_000254738.1
Coordinates 1044443..1044778 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A1V2T5M9
Locus tag HUZ45_RS05125 Protein ID WP_000581941.1
Coordinates 1044195..1044443 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ45_RS05110 (1039314) 1039314..1040477 - 1164 Protein_1006 histidine kinase dimerization/phospho-acceptor domain-containing protein -
HUZ45_RS05115 (1040534) 1040534..1041835 + 1302 WP_023281448.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
HUZ45_RS05120 (1041883) 1041883..1044117 + 2235 WP_000226815.1 GTP pyrophosphokinase -
HUZ45_RS05125 (1044195) 1044195..1044443 + 249 WP_000581941.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
HUZ45_RS05130 (1044443) 1044443..1044778 + 336 WP_000254738.1 endoribonuclease MazF Toxin
HUZ45_RS05135 (1044849) 1044849..1045640 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
HUZ45_RS05140 (1045868) 1045868..1047505 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
HUZ45_RS05145 (1047593) 1047593..1048891 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12098.04 Da        Isoelectric Point: 8.2618

>T161798 WP_000254738.1 NZ_CP055181:1044443-1044778 [Shigella flexneri]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T161798 NZ_CP071305:c2139232-2138975 [Streptococcus suis]
ATGGGAATCCATTTTACAGAGGATGCTTGGGAAGACTATCTTTATTGGCAAGGTCAGGATAAGAAAATACTGAAGAGAAT
CAATAAGATTATCAAGGATATGCAACGACATCCTTTTGAAGGCATTGCCAAACCAGAACCTTTGAAGTATGATTATCAAG
GTGCTTGGTCAAGAAGGATTGATGCGGAGAATCGGTTGATTTATATGGTGGAGGCTGATCGGTTGTGCATCCTGTCGCTA
AAGGACCATTATGGGTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9385.76 Da        Isoelectric Point: 4.4665

>AT161798 WP_000581941.1 NZ_CP055181:1044195-1044443 [Shigella flexneri]
MIHSSVKRWGNSPAVRIPTTLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT161798 NZ_CP071305:c2139494-2139234 [Streptococcus suis]
ATGGAAGCTATTGTATATTCCCATTTTCGAAATCACTTAAAGGACTATATGAAAAAGGTCAATGACGAGTTTGAACCTTT
GGTCGTGGTTAATAAAAATCCAGAGGAAGATATTGTTGTACTCTCAAAGAGCGAGTGGGATAGTTTACAAGAAACCCTCG
CTGTTGCTCGGAACACCTATCTGTCTCAAAAAGTTTTGCGTGGTATGGCTCAAGTCAAAGCAGGGCAAACCCAAGAACGA
AACCTGATAGAGGCCGACTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CK9
PDB 5CKH
PDB 5CO7
PDB 3NFC
PDB 5CR2
PDB 5CKB
PDB 5CQY
PDB 5CKD
PDB 5CKE
PDB 5CKF

Antitoxin

Source ID Structure
AlphaFold DB A0A1V2T5M9

References