Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 905972..906626 Replicon chromosome
Accession NZ_CP055181
Organism Shigella flexneri strain STEFF_17

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag HUZ45_RS04495 Protein ID WP_000244777.1
Coordinates 906219..906626 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HUZ45_RS04490 Protein ID WP_000354046.1
Coordinates 905972..906238 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ45_RS04465 (901141) 901141..901884 + 744 WP_000951962.1 SDR family oxidoreductase -
HUZ45_RS04470 (901941) 901941..903374 - 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
HUZ45_RS04475 (903419) 903419..903730 + 312 WP_122056851.1 N(4)-acetylcytidine aminohydrolase -
HUZ45_RS04480 (903894) 903894..904553 + 660 WP_000250274.1 hemolysin III family protein -
HUZ45_RS04485 (904749) 904749..905729 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
HUZ45_RS04490 (905972) 905972..906238 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HUZ45_RS04495 (906219) 906219..906626 + 408 WP_000244777.1 protein YgfX Toxin
HUZ45_RS04500 (906666) 906666..907187 - 522 WP_001055874.1 flavodoxin FldB -
HUZ45_RS04505 (907299) 907299..908195 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HUZ45_RS04510 (908220) 908220..908930 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HUZ45_RS04515 (908936) 908936..910669 + 1734 WP_000813201.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T161797 WP_000244777.1 NZ_CP055181:906219-906626 [Shigella flexneri]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T161797 NZ_CP071305:c1431733-1431464 [Streptococcus suis]
ATGGCTTATAAACTTGTTCTCAGCGATGATGCTTTGAAACAGTTGAAAAAAATGGACAGGCATGTGGGGATGATGCTTGC
TAAGGATTTGAAAAAACGTTTGGACGGTTTGGAAAATCCAAGACAGTTTGGCAAGGCCTTGGTTGGAGATTATAAGGGCC
TGTGGCGGTATCGTGTGGGAAATTACCGTGTGATTTGCGATATTATTGATAACAAAATGGTCATCCTAGCCCTTGAAATC
GGGTACCGGAAAGAAATCTATAAAAAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT161797 WP_000354046.1 NZ_CP055181:905972-906238 [Shigella flexneri]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT161797 NZ_CP071305:c1431950-1431723 [Streptococcus suis]
ATGGGAATTGTATCCTTGCGGTTAAACGATGCAGAAGAAGAAATCTTCAGAAGCTATGCCATTCATACAGGAAAAAGCCT
GTCTGAGTTATTTAAAACTGCACTTGCTGAACAGATAGAAGACCAGTTGGACTATGAAATTGGTATCAAGGCTTTGAAGA
AATTCCAAGAAAATCCTGTTACACACTCGATTGATGATGTTCTTGCGGAGTTAGAAGATGGCTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References