Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 18288..18931 Replicon plasmid unnamed1
Accession NZ_CP055180
Organism Shigella flexneri strain STEFF_18

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag HUZ46_RS23650 Protein ID WP_001044768.1
Coordinates 18515..18931 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag HUZ46_RS23645 Protein ID WP_001261287.1
Coordinates 18288..18518 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ46_RS23625 (13448) 13448..14536 + 1089 WP_000952217.1 transcriptional repressor PifC -
HUZ46_RS23630 (14538) 14538..16763 + 2226 WP_000698737.1 P-loop NTPase fold protein -
HUZ46_RS23635 (16813) 16813..17712 - 900 WP_000963206.1 nucleotide-binding protein -
HUZ46_RS23640 (17702) 17702..17992 - 291 WP_000111771.1 hypothetical protein -
HUZ46_RS23645 (18288) 18288..18518 + 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
HUZ46_RS23650 (18515) 18515..18931 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
HUZ46_RS23655 (19093) 19093..21231 - 2139 WP_000350638.1 AAA family ATPase -
HUZ46_RS23660 (21585) 21585..21842 + 258 WP_000343085.1 hypothetical protein -
HUZ46_RS23665 (21842) 21842..22432 + 591 WP_000194575.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(3')-Ia / dfrA14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / blaCTX-M-55 / sitABCD iutA / iucD / iucC / iucB / iucA 1..122007 122007


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T161784 WP_001044768.1 NZ_CP055180:18515-18931 [Shigella flexneri]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T161784 NZ_CP071279:c4486372-4486088 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT161784 WP_001261287.1 NZ_CP055180:18288-18518 [Shigella flexneri]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT161784 NZ_CP071279:c4486604-4486362 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References