Detailed information of TA system TA161781

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4323729..4324324
• Replicon: chromosome
• Accession: NZ_CP055179
• Organism: Shigella flexneri strain STEFF_18

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: HUZ46_RS20835
• Protein ID: WP_000239581.1
• Coordinates: 4323729..4324079 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: HUZ46_RS20840
• Protein ID: WP_001223213.1
• Coordinates: 4324073..4324324 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ46_RS20815 (4319183)	4319183..4320205	-	1023	WP_063625321.1	ABC transporter permease	-
HUZ46_RS20820 (4320219)	4320219..4321721	-	1503	WP_063625322.1	sugar ABC transporter ATP-binding protein	-
HUZ46_RS20825 (4321854)	4321854..4322810	-	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
HUZ46_RS20830 (4323120)	4323120..4323650	+	531	WP_000055075.1	inorganic diphosphatase	-
HUZ46_RS20835 (4323729)	4323729..4324079	-	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
HUZ46_RS20840 (4324073)	4324073..4324324	-	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
HUZ46_RS20845 (4324536)	4324536..4324877	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
HUZ46_RS20850 (4324880)	4324880..4328659	-	3780	WP_063625323.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T161781 WP_000239581.1 NZ_CP055179:c4324079-4323729 [Shigella flexneri]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T161781 NZ_CP071279:c3657198-3656881 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT161781 WP_001223213.1 NZ_CP055179:c4324324-4324073 [Shigella flexneri]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT161781 NZ_CP071279:c3656888-3656601 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCAACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7