Detailed information of TA system TA161780

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 4312385..4312907
• Replicon: chromosome
• Accession: NZ_CP055179
• Organism: Shigella flexneri strain STEFF_18

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: HUZ46_RS20775
• Protein ID: WP_104773392.1
• Coordinates: 4312385..4312675 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0SC69
• Locus tag: HUZ46_RS20780
• Protein ID: WP_000212715.1
• Coordinates: 4312665..4312907 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ46_RS20760 (4307553)	4307553..4309208	+	1656	WP_063625319.1	alpha,alpha-phosphotrehalase	-
HUZ46_RS20765 (4309602)	4309602..4311740	+	2139	WP_000187798.1	anaerobic ribonucleoside-triphosphate reductase	-
HUZ46_RS20770 (4311920)	4311920..4312384	+	465	WP_001009182.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
HUZ46_RS20775 (4312385)	4312385..4312675	-	291	WP_104773392.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUZ46_RS20780 (4312665)	4312665..4312907	-	243	WP_000212715.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
HUZ46_RS20785 (4313099)	4313099..4313485	-	387	WP_001232253.1	cytochrome b562	-
HUZ46_RS20790 (4313541)	4313541..4314893	-	1353	WP_063625320.1	metalloprotease PmbA	-
HUZ46_RS20795 (4314987)	4314987..4315538	+	552	WP_000166270.1	ribosome biogenesis factor YjgA	-
HUZ46_RS20800 (4315621)	4315621..4316994	-	1374	WP_001219792.1	UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-82)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-72)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11367.23 Da
• Isoelectric Point: 10.0238

>T161780 WP_104773392.1 NZ_CP055179:c4312675-4312385 [Shigella flexneri]
MNYELAFDPRALKEWQKLGVAVREQFKKKLEDVLKRPRNPSAKLRDLPDCYKIKLRTQGYRLVYQVNDKELLVLVITIGK
RENSAVYEDATKRLDE

Nucleotide

• Length: 291 bp

>T161780 NZ_CP055179:c4312675-4312385 [Shigella flexneri]
ATGAACTATGAGCTGGCGTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGGCAGTCCGGGAACAATTTAA
AAAAAAGCTTGAGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTGCGGGATTTACCGGACTGCTACAAAATTA
AGCTGCGCACACAGGGATATCGCCTGGTTTATCAGGTTAACGATAAAGAGTTGCTCGTGTTGGTGATAACTATTGGTAAG
AGAGAGAACTCAGCCGTTTATGAAGACGCGACTAAACGGCTGGATGAGTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9125.56 Da
• Isoelectric Point: 5.1369

>AT161780 WP_000212715.1 NZ_CP055179:c4312907-4312665 [Shigella flexneri]
MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAENGRMPVKTLTVSDSEEALLRTVRERLANPQEAIKVRLDEL

Nucleotide

• Length: 243 bp

>AT161780 NZ_CP055179:c4312907-4312665 [Shigella flexneri]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATCACGCC
TACTGAAGCAGTGCGACTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAAGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9H4B3