Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4176832..4177667 Replicon chromosome
Accession NZ_CP055179
Organism Shigella flexneri strain STEFF_18

Toxin (Protein)


Gene name yeeV Uniprot ID A0A1M0VTI9
Locus tag HUZ46_RS20090 Protein ID WP_072649755.1
Coordinates 4176832..4177209 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID B7LDN9
Locus tag HUZ46_RS20095 Protein ID WP_001285607.1
Coordinates 4177299..4177667 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ46_RS20065 (4172332) 4172332..4172511 + 180 Protein_3937 peptidase -
HUZ46_RS20070 (4172910) 4172910..4174946 - 2037 WP_239612939.1 hypothetical protein -
HUZ46_RS20075 (4175902) 4175902..4176045 - 144 Protein_3939 hypothetical protein -
HUZ46_RS20080 (4176130) 4176130..4176327 - 198 WP_001327226.1 DUF957 domain-containing protein -
HUZ46_RS20085 (4176347) 4176347..4176835 - 489 WP_000761685.1 DUF5983 family protein -
HUZ46_RS20090 (4176832) 4176832..4177209 - 378 WP_072649755.1 TA system toxin CbtA family protein Toxin
HUZ46_RS20095 (4177299) 4177299..4177667 - 369 WP_001285607.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
HUZ46_RS20100 (4177747) 4177747..4177968 - 222 WP_000692350.1 DUF987 domain-containing protein -
HUZ46_RS20105 (4178055) 4178055..4178531 - 477 WP_001384029.1 RadC family protein -
HUZ46_RS20110 (4178546) 4178546..4179031 - 486 WP_000213722.1 antirestriction protein -
HUZ46_RS20115 (4179123) 4179123..4179941 - 819 WP_001175175.1 DUF932 domain-containing protein -
HUZ46_RS20120 (4180031) 4180031..4180240 - 210 WP_032150870.1 DUF905 family protein -
HUZ46_RS20125 (4180270) 4180270..4180947 - 678 WP_001097301.1 hypothetical protein -
HUZ46_RS20130 (4181095) 4181095..4181775 - 681 WP_001282919.1 WYL domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / floR fimC / fimI / fimA / fimE / fimB / papI / papB 4162793..4275853 113060


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(19-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14216.18 Da        Isoelectric Point: 7.3523

>T161779 WP_072649755.1 NZ_CP055179:c4177209-4176832 [Shigella flexneri]
MNTLPDTHVREASHCQSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SVCTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPWAKQ

Download         Length: 378 bp

>T161779 NZ_CP071279:1698367-1698469 [Klebsiella pneumoniae subsp. pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13689.53 Da        Isoelectric Point: 6.4669

>AT161779 WP_001285607.1 NZ_CP055179:c4177667-4177299 [Shigella flexneri]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKRLTCEADTLGSCGYVYMAVYPTPETKK

Download         Length: 369 bp

>AT161779 NZ_CP071279:c1698476-1698331 [Klebsiella pneumoniae subsp. pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1M0VTI9

Antitoxin

Source ID Structure
AlphaFold DB A0A1M0JLU8

References