Detailed information of TA system TA161777

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3586866..3587703
• Replicon: chromosome
• Accession: NZ_CP055179
• Organism: Shigella flexneri strain STEFF_18

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: HUZ46_RS17440
• Protein ID: WP_000227784.1
• Coordinates: 3587161..3587703 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: HUZ46_RS17435
• Protein ID: WP_001297137.1
• Coordinates: 3586866..3587177 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ46_RS17410 (3581886)	3581886..3582833	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
HUZ46_RS17415 (3582855)	3582855..3584846	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
HUZ46_RS17420 (3584836)	3584836..3585450	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
HUZ46_RS17425 (3585450)	3585450..3585779	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
HUZ46_RS17430 (3585791)	3585791..3586681	+	891	WP_000971336.1	heme o synthase	-
HUZ46_RS17435 (3586866)	3586866..3587177	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
HUZ46_RS17440 (3587161)	3587161..3587703	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
HUZ46_RS17445 (3587759)	3587759..3588694	-	936	WP_001297127.1	tetratricopeptide repeat protein	-
HUZ46_RS17450 (3589102)	3589102..3590466	+	1365	WP_001000974.1	MFS transporter	-
HUZ46_RS17455 (3590594)	3590594..3591085	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
HUZ46_RS17460 (3591253)	3591253..3592164	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T161777 WP_000227784.1 NZ_CP055179:3587161-3587703 [Shigella flexneri]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T161777 NZ_CP071279:11160-11528 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT161777 WP_001297137.1 NZ_CP055179:3586866-3587177 [Shigella flexneri]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT161777 NZ_CP071279:10942-11163 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3