Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3552786..3553404 Replicon chromosome
Accession NZ_CP055179
Organism Shigella flexneri strain STEFF_18

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HUZ46_RS17270 Protein ID WP_001291435.1
Coordinates 3553186..3553404 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HUZ46_RS17265 Protein ID WP_000344800.1
Coordinates 3552786..3553160 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ46_RS17255 (3547875) 3547875..3549068 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HUZ46_RS17260 (3549091) 3549091..3552240 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
HUZ46_RS17265 (3552786) 3552786..3553160 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HUZ46_RS17270 (3553186) 3553186..3553404 + 219 WP_001291435.1 HHA domain-containing protein Toxin
HUZ46_RS17275 (3553576) 3553576..3554127 + 552 WP_000102564.1 maltose O-acetyltransferase -
HUZ46_RS17280 (3554243) 3554243..3554713 + 471 WP_000136192.1 YlaC family protein -
HUZ46_RS17285 (3554877) 3554877..3556427 + 1551 WP_063625273.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HUZ46_RS17290 (3556469) 3556469..3556822 - 354 WP_000878140.1 DUF1428 domain-containing protein -
HUZ46_RS17300 (3557201) 3557201..3557512 + 312 WP_000409911.1 MGMT family protein -
HUZ46_RS17305 (3557543) 3557543..3558115 - 573 WP_000779826.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T161776 WP_001291435.1 NZ_CP055179:3553186-3553404 [Shigella flexneri]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T161776 NZ_CP071276:c1350735-1349989 [Bacillus subtilis]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATCGTGGCCGGACTGGGGTTATACGTGTATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTCCGCCATCCGGAAAACTTGGTATTTGCTGTTTGTTCTGGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGAACGGTATCTCATTGTCGCAGTCAGTTTTGCTTTGATTGATGCTTTT
ATCTTCTTAAGTGCATACGTCAAAAAACTGGCCGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTACCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAACCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGTATTGATAAACTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCCTCTCTT
TGCCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAGTGGTTCTCATCCCCTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT161776 WP_000344800.1 NZ_CP055179:3552786-3553160 [Shigella flexneri]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT161776 NZ_CP071276:c1349989-1349819 [Bacillus subtilis]
ATGGAACGTGCGTTTCAAAACAGATGCGAGCCCAGAGCGGCGAAGCCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCATACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References