Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1309248..1309873 Replicon chromosome
Accession NZ_CP055179
Organism Shigella flexneri strain STEFF_18

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag HUZ46_RS06300 Protein ID WP_000911330.1
Coordinates 1309475..1309873 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag HUZ46_RS06295 Protein ID WP_000450524.1
Coordinates 1309248..1309475 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ46_RS06270 (1305051) 1305051..1305521 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
HUZ46_RS06275 (1305521) 1305521..1306093 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
HUZ46_RS06280 (1306239) 1306239..1307117 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
HUZ46_RS06285 (1307134) 1307134..1308168 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
HUZ46_RS06290 (1308381) 1308381..1309094 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
HUZ46_RS06295 (1309248) 1309248..1309475 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
HUZ46_RS06300 (1309475) 1309475..1309873 + 399 WP_000911330.1 type II toxin-antitoxin system VapC family toxin Toxin
HUZ46_RS06305 (1310020) 1310020..1310883 + 864 WP_001267507.1 neutral zinc metallopeptidase -
HUZ46_RS06310 (1310898) 1310898..1312913 + 2016 WP_000829335.1 tRNA cytosine(34) acetyltransferase TmcA -
HUZ46_RS06315 (1312987) 1312987..1313685 + 699 WP_000679812.1 esterase -
HUZ46_RS06320 (1313795) 1313795..1313995 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14894.25 Da        Isoelectric Point: 9.2216

>T161766 WP_000911330.1 NZ_CP055179:1309475-1309873 [Shigella flexneri]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T161766 NZ_CP071265:850812-851219 [Citrobacter freundii]
GTGGTCCAGTGGCAATCTGATTTACGCGTATCATGGCGCGCGCAGTGGCTTTCACTGCTAATTCATGGGCTGGTCGCGGT
GTTTATTTTATTGATGCCCTGGCCATTGAGCTACACCCCATTATGGCTGGTTTTGCTCTCTCTGGTGGTATTCGATAGCG
TTCGCAGTCAGCGTCGAATCAACGCCTGTCAGGGTGAGATTCGGCTGTTGATGGATGGTCGTCTGCGCTGGCAAGGGCAA
GAGTGGTCTATCGTTAGCGCCCCCTGGATGGTTAAAACAGGCATGATGCTGCGTTTGCGCTCTGATGCCGGAAAACGTCA
ACATTTGTGGCTGGCGGCAGACAGTATGGATGATGCTGAGTGGCGGGATTTACGGCGGTTGATGCTGCAAAAGGCGAAGC
AGAAGTAG

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT161766 WP_000450524.1 NZ_CP055179:1309248-1309475 [Shigella flexneri]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT161766 NZ_CP071265:850565-850831 [Citrobacter freundii]
ATGGATATTAACAACAAAGCACGTATTCATTGGGCATGCCGCCGCGGTATGCGCGAGCTGGACATCTCGATCATGCCGTT
TTTCGAACATGAATATGACAGCTTAAGTGACGAAGAAAAACGTATCTTTATCCGTCTGCTAGAATGTGATGATCCAGATT
TATTTAACTGGTTGATGAATCATGGGAAACCAGCTGATGCTGAGCTGGAGCAGATGGTGCGACTTATCCAGACACGGAAT
CGGGAACGTGGTCCAGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References