Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 324691..325491 Replicon chromosome
Accession NZ_CP055179
Organism Shigella flexneri strain STEFF_18

Toxin (Protein)


Gene name ataT Uniprot ID -
Locus tag HUZ46_RS01480 Protein ID WP_000342451.1
Coordinates 324964..325491 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag HUZ46_RS01475 Protein ID WP_001277108.1
Coordinates 324691..324957 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ46_RS01455 (320349) 320349..321017 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
HUZ46_RS01460 (321010) 321010..322068 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
HUZ46_RS01465 (322312) 322312..323166 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
HUZ46_RS01470 (323438) 323438..324541 + 1104 WP_078167906.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
HUZ46_RS01475 (324691) 324691..324957 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
HUZ46_RS01480 (324964) 324964..325491 + 528 WP_000342451.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
HUZ46_RS01485 (325488) 325488..325871 - 384 WP_000778795.1 aspartate 1-decarboxylase autocleavage activator PanM -
HUZ46_RS01490 (326295) 326295..327404 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
HUZ46_RS01495 (327452) 327452..328378 + 927 WP_000003004.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
HUZ46_RS01500 (328375) 328375..329652 + 1278 WP_000803798.1 branched chain amino acid ABC transporter permease LivM -
HUZ46_RS01505 (329649) 329649..330416 + 768 WP_000082110.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19749.73 Da        Isoelectric Point: 7.3232

>T161761 WP_000342451.1 NZ_CP055179:324964-325491 [Shigella flexneri]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAELPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T161761 NZ_CP071263:c5175049-5174942 [Escherichia coli]
ATGACGCTCACGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT161761 WP_001277108.1 NZ_CP055179:324691-324957 [Shigella flexneri]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT161761 NZ_CP071263:5175097-5175163 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTATACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References