Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 62490..63015 Replicon plasmid unnamed2
Accession NZ_CP055176
Organism Klebsiella pneumoniae strain STEFF_19

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag HUZ47_RS27575 Protein ID WP_013023785.1
Coordinates 62710..63015 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag HUZ47_RS27570 Protein ID WP_001568025.1
Coordinates 62490..62708 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ47_RS27530 (HUZ47_27490) 57707..57925 - 219 WP_021312527.1 hypothetical protein -
HUZ47_RS27535 (HUZ47_27495) 57989..58234 - 246 WP_065905238.1 hypothetical protein -
HUZ47_RS27540 (HUZ47_27500) 58269..58685 - 417 WP_021312475.1 type II toxin-antitoxin system VapC family toxin -
HUZ47_RS27545 (HUZ47_27505) 58682..58912 - 231 WP_021312476.1 type II toxin-antitoxin system VapB family antitoxin -
HUZ47_RS27550 (HUZ47_27510) 59188..59688 + 501 WP_021312477.1 HEPN family nuclease -
HUZ47_RS27555 (HUZ47_27515) 59701..60477 + 777 WP_021312478.1 hypothetical protein -
HUZ47_RS27560 (HUZ47_27520) 60639..60887 + 249 WP_023332841.1 hypothetical protein -
HUZ47_RS27565 60941..61927 - 987 WP_021312480.1 hypothetical protein -
HUZ47_RS27570 (HUZ47_27530) 62490..62708 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HUZ47_RS27575 (HUZ47_27535) 62710..63015 + 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
HUZ47_RS27580 (HUZ47_27540) 63202..64206 + 1005 WP_001568027.1 hypothetical protein -
HUZ47_RS27585 (HUZ47_27545) 64390..65169 + 780 WP_001568028.1 site-specific integrase -
HUZ47_RS27590 (HUZ47_27550) 65227..65484 - 258 WP_000764642.1 hypothetical protein -
HUZ47_RS27595 (HUZ47_27555) 65613..65726 - 114 WP_014343462.1 hypothetical protein -
HUZ47_RS27600 (HUZ47_27560) 66357..67112 + 756 WP_001568031.1 replication initiation protein RepE -
HUZ47_RS27605 (HUZ47_27565) 67402..67836 - 435 WP_021312407.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - mrkA / mrkB / mrkC / mrkD / mrkF / mrkJ 1..136415 136415


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T161760 WP_013023785.1 NZ_CP055176:62710-63015 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T161760 NZ_CP071263:c4260090-4259872 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT161760 WP_001568025.1 NZ_CP055176:62490-62708 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT161760 NZ_CP071263:c4260490-4260116 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References