Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5303521..5304146 Replicon chromosome
Accession NZ_CP055174
Organism Klebsiella pneumoniae strain STEFF_19

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HUZ47_RS25605 Protein ID WP_002882817.1
Coordinates 5303521..5303904 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HUZ47_RS25610 Protein ID WP_004150355.1
Coordinates 5303904..5304146 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ47_RS25590 (HUZ47_25555) 5300887..5301789 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HUZ47_RS25595 (HUZ47_25560) 5301786..5302421 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HUZ47_RS25600 (HUZ47_25565) 5302418..5303347 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HUZ47_RS25605 (HUZ47_25570) 5303521..5303904 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HUZ47_RS25610 (HUZ47_25575) 5303904..5304146 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
HUZ47_RS25615 (HUZ47_25580) 5304351..5305268 + 918 WP_004895173.1 alpha/beta hydrolase -
HUZ47_RS25620 (HUZ47_25585) 5305282..5306223 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HUZ47_RS25625 (HUZ47_25590) 5306268..5306705 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
HUZ47_RS25630 (HUZ47_25595) 5306702..5307562 - 861 WP_004146232.1 virulence factor BrkB family protein -
HUZ47_RS25635 (HUZ47_25600) 5307556..5308155 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T161755 WP_002882817.1 NZ_CP055174:c5303904-5303521 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T161755 NZ_CP071263:c2767760-2767575 [Escherichia coli]
GTGAAAAGTGCAGACGTGATCGCCATCCTGAAGCAGCATGGCTGGGAACATATTCGAACCCGTGGCAGTCATCATCAGTT
CCGCCACCCTGTTCATCCGGGTCTGGTGACGGTTCCGCACCCTAAAAAAGATATTAAGCCCGGTACGCTGGCGCAAATTG
GGCGTCAGGCTGGTTTAACATTTTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT161755 WP_004150355.1 NZ_CP055174:c5304146-5303904 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT161755 NZ_CP071263:c2767558-2767148 [Escherichia coli]
ATGTTTTATCCCGCCTATATCCACAGTGATCCTGACGGCAGCGCCAGCGGTTTTTTCCCTGATGTTCCAGGCTGTTATTT
TGCTGGTGATACGCTGGACTACGCTTTCCAGGATGCGCGAAGTGCACTGGTCGCGCACTTCGAAACCTTATGCGAGATGA
ATGAAGAGTTGCCGCTTCCAGGCTCTGTGGAAACACATCTGGTCCAGCGTGCGCAGGACTTTATTGGCGGGCAATGGTTG
CTGGTGGATATCAATATGAATCAGTTCGAAGGTCGGGCTGAACGTATCAATATTACGATGCCAAAAAGATTACTGAACAA
GATTGATACCTACGTGCGCAATAACCCAGACTATGCCAACAGAAGTGCGTTTCTGGCGGAAGCTGCCCGGCGTGTATTGC
CGGGCGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References