Detailed information of TA system TA161751

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 4056827..4057446
• Replicon: chromosome
• Accession: NZ_CP055174
• Organism: Klebsiella pneumoniae strain STEFF_19

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: R8WYV2
• Locus tag: HUZ47_RS19585
• Protein ID: WP_002892050.1
• Coordinates: 4057228..4057446 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: HUZ47_RS19580
• Protein ID: WP_064151478.1
• Coordinates: 4056827..4057201 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ47_RS19570 (HUZ47_19525)	4051979..4053172	+	1194	WP_004177236.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
HUZ47_RS19575 (HUZ47_19530)	4053195..4056341	+	3147	WP_002892069.1	multidrug efflux RND transporter permease subunit AcrB	-
HUZ47_RS19580 (HUZ47_19535)	4056827..4057201	+	375	WP_064151478.1	Hha toxicity modulator TomB	Antitoxin
HUZ47_RS19585 (HUZ47_19540)	4057228..4057446	+	219	WP_002892050.1	HHA domain-containing protein	Toxin
HUZ47_RS19590 (HUZ47_19545)	4057605..4058171	+	567	WP_002892030.1	maltose O-acetyltransferase	-
HUZ47_RS19595 (HUZ47_19550)	4058143..4058283	-	141	WP_032409038.1	hypothetical protein	-
HUZ47_RS19600 (HUZ47_19555)	4058304..4058774	+	471	WP_002892026.1	YlaC family protein	-
HUZ47_RS19605 (HUZ47_19560)	4058749..4060200	-	1452	WP_002892023.1	PLP-dependent aminotransferase family protein	-
HUZ47_RS19610 (HUZ47_19565)	4060301..4060999	+	699	WP_004893822.1	GNAT family protein	-
HUZ47_RS19615 (HUZ47_19570)	4060996..4061136	-	141	WP_002892018.1	type B 50S ribosomal protein L36	-
HUZ47_RS19620 (HUZ47_19575)	4061136..4061399	-	264	WP_002892011.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8628.00 Da
• Isoelectric Point: 8.9008

>T161751 WP_002892050.1 NZ_CP055174:4057228-4057446 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Nucleotide

• Length: 219 bp

>T161751 NZ_CP071263:2291023-2291487 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCTTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGGCAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14340.00 Da
• Isoelectric Point: 4.8989

>AT161751 WP_064151478.1 NZ_CP055174:4056827-4057201 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFANASRENPASLSC

Nucleotide

• Length: 375 bp

>AT161751 NZ_CP071263:2290688-2291023 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A2P8K6F2