Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/ParE-YafN |
| Location | 53017..53552 | Replicon | plasmid unnamed1 |
| Accession | NZ_CP055171 | ||
| Organism | Shigella flexneri strain STEFF_22 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | Q3YTD6 |
| Locus tag | HUZ49_RS23455 | Protein ID | WP_000222766.1 |
| Coordinates | 53017..53304 (-) | Length | 96 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | V0VIP2 |
| Locus tag | HUZ49_RS23460 | Protein ID | WP_001132900.1 |
| Coordinates | 53301..53552 (-) | Length | 84 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HUZ49_RS23425 (48565) | 48565..49425 | - | 861 | WP_000557454.1 | aminoglycoside N-acetyltransferase AAC(3)-IId | - |
| HUZ49_RS23430 (49568) | 49568..50062 | + | 495 | Protein_56 | IS4-like element ISVsa5 family transposase | - |
| HUZ49_RS23435 (50116) | 50116..50820 | - | 705 | WP_001067858.1 | IS6-like element IS26 family transposase | - |
| HUZ49_RS23440 (50888) | 50888..52186 | + | 1299 | Protein_58 | Tn3-like element Tn3 family transposase | - |
| HUZ49_RS23445 (52220) | 52220..52720 | + | 501 | Protein_59 | Tn3 family transposase | - |
| HUZ49_RS23450 (52754) | 52754..52942 | + | 189 | Protein_60 | IS3 family transposase | - |
| HUZ49_RS23455 (53017) | 53017..53304 | - | 288 | WP_000222766.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| HUZ49_RS23460 (53301) | 53301..53552 | - | 252 | WP_001132900.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | Antitoxin |
| HUZ49_RS23465 (54518) | 54518..55375 | - | 858 | WP_039002220.1 | incFII family plasmid replication initiator RepA | - |
| HUZ49_RS23470 (55368) | 55368..55442 | - | 75 | WP_061357229.1 | RepA leader peptide Tap | - |
| HUZ49_RS23475 (55674) | 55674..55931 | - | 258 | WP_001541895.1 | replication regulatory protein RepA | - |
| HUZ49_RS23480 (56199) | 56199..56348 | - | 150 | WP_001312851.1 | Hok/Gef family protein | - |
| - (56392) | 56392..56453 | + | 62 | NuclAT_1 | - | - |
| - (56392) | 56392..56453 | + | 62 | NuclAT_1 | - | - |
| - (56392) | 56392..56453 | + | 62 | NuclAT_1 | - | - |
| - (56392) | 56392..56453 | + | 62 | NuclAT_1 | - | - |
| HUZ49_RS23485 (56795) | 56795..57007 | - | 213 | WP_001541896.1 | ANR family transcriptional regulator | - |
| HUZ49_RS23490 (57142) | 57142..57702 | - | 561 | WP_000139310.1 | fertility inhibition protein FinO | - |
| HUZ49_RS23495 (57757) | 57757..58500 | - | 744 | WP_039002216.1 | conjugal transfer pilus acetylase TraX | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | tet(M) / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 / floR / sul2 / tet(A) / aph(3')-Ia / sul3 / blaTEM-1B / lnu(F) / aac(3)-IId | - | 1..131772 | 131772 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 96 a.a. Molecular weight: 11147.23 Da Isoelectric Point: 10.5832
>T161728 WP_000222766.1 NZ_CP055171:c53304-53017 [Shigella flexneri]
MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR
MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR
Download Length: 288 bp
>T161728 NZ_CP071259:c4960698-4960387 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA
Antitoxin
Download Length: 84 a.a. Molecular weight: 9211.41 Da Isoelectric Point: 4.0780
>AT161728 WP_001132900.1 NZ_CP055171:c53552-53301 [Shigella flexneri]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL
Download Length: 252 bp
>AT161728 NZ_CP071259:c4960386-4960096 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829CDZ2 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | V0VIP2 |