Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4276703..4277289 Replicon chromosome
Accession NZ_CP055170
Organism Shigella flexneri strain STEFF_22

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag HUZ49_RS20635 Protein ID WP_042014162.1
Coordinates 4277059..4277289 (-) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag HUZ49_RS20630 Protein ID WP_000593555.1
Coordinates 4276703..4277062 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ49_RS20605 (4272835) 4272835..4273779 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
HUZ49_RS20610 (4273776) 4273776..4274609 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
HUZ49_RS20615 (4274609) 4274609..4275373 + 765 WP_032218476.1 nickel import ATP-binding protein NikD -
HUZ49_RS20620 (4275370) 4275370..4276176 + 807 WP_000173666.1 nickel import ATP-binding protein NikE -
HUZ49_RS20625 (4276182) 4276182..4276583 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
HUZ49_RS20630 (4276703) 4276703..4277062 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
HUZ49_RS20635 (4277059) 4277059..4277289 - 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
HUZ49_RS20640 (4277393) 4277393..4278517 - 1125 WP_001314210.1 ABC transporter permease -
HUZ49_RS20645 (4278517) 4278517..4281252 - 2736 WP_000149158.1 ribosome-associated ATPase/putative transporter RbbA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T161726 WP_042014162.1 NZ_CP055170:c4277289-4277059 [Shigella flexneri]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T161726 NZ_CP071259:4670774-4671148 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT161726 WP_000593555.1 NZ_CP055170:c4277062-4276703 [Shigella flexneri]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT161726 NZ_CP071259:4670316-4670684 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References