Detailed information of TA system TA161724

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3844598..3845433
• Replicon: chromosome
• Accession: NZ_CP055170
• Organism: Shigella flexneri strain STEFF_22

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A3P1YKV1
• Locus tag: HUZ49_RS18455
• Protein ID: WP_016240657.1
• Coordinates: 3844598..3844975 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HUZ49_RS18460
• Protein ID: WP_113446226.1
• Coordinates: 3845065..3845433 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ49_RS18425 (3840248)	3840248..3842089	+	1842	WP_000437371.1	RNA polymerase sigma factor RpoD	-
HUZ49_RS18430 (3842168)	3842168..3842674	-	507	WP_000228937.1	G/U mismatch-specific DNA glycosylase	-
HUZ49_RS18440 (3842974)	3842974..3843819	-	846	WP_049038750.1	DUF4942 domain-containing protein	-
HUZ49_RS18445 (3843904)	3843904..3844101	-	198	WP_063080560.1	DUF957 domain-containing protein	-
HUZ49_RS18450 (3844113)	3844113..3844601	-	489	WP_063080559.1	DUF5983 family protein	-
HUZ49_RS18455 (3844598)	3844598..3844975	-	378	WP_016240657.1	TA system toxin CbtA family protein	Toxin
HUZ49_RS18460 (3845065)	3845065..3845433	-	369	WP_113446226.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HUZ49_RS18465 (3845513)	3845513..3845734	-	222	WP_000692312.1	DUF987 domain-containing protein	-
HUZ49_RS18470 (3845803)	3845803..3846279	-	477	WP_001186742.1	RadC family protein	-
HUZ49_RS18475 (3846294)	3846294..3846779	-	486	WP_042631074.1	antirestriction protein	-
HUZ49_RS18480 (3846870)	3846870..3847688	-	819	WP_001234702.1	DUF932 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14192.16 Da
• Isoelectric Point: 8.2905

>T161724 WP_016240657.1 NZ_CP055170:c3844975-3844598 [Shigella flexneri]
MKTLPDTHVREASRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAKQ

Nucleotide

• Length: 378 bp

>T161724 NZ_CP071259:3977324-3977602 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13663.39 Da
• Isoelectric Point: 6.7388

>AT161724 WP_113446226.1 NZ_CP055170:c3845433-3845065 [Shigella flexneri]
VSDTFSGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLTDRAGIRGRFSDADANHLDQAFPLLMKQLELMLTSS
ELNPHRQNTVTLYVKGLTCHADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT161724 NZ_CP071259:3977061-3977321 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: A0A3P1YKV1