Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3641234..3641888 Replicon chromosome
Accession NZ_CP055170
Organism Shigella flexneri strain STEFF_22

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag HUZ49_RS17445 Protein ID WP_000244772.1
Coordinates 3641234..3641641 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HUZ49_RS17450 Protein ID WP_000354046.1
Coordinates 3641622..3641888 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ49_RS17425 (3637191) 3637191..3638924 - 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -
HUZ49_RS17430 (3638930) 3638930..3639640 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HUZ49_RS17435 (3639665) 3639665..3640561 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HUZ49_RS17440 (3640673) 3640673..3641194 + 522 WP_001055874.1 flavodoxin FldB -
HUZ49_RS17445 (3641234) 3641234..3641641 - 408 WP_000244772.1 protein YgfX Toxin
HUZ49_RS17450 (3641622) 3641622..3641888 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HUZ49_RS17455 (3642131) 3642131..3643111 + 981 WP_063108291.1 tRNA-modifying protein YgfZ -
HUZ49_RS17460 (3643307) 3643307..3643966 - 660 WP_000250274.1 hemolysin III family protein -
HUZ49_RS17465 (3644130) 3644130..3644441 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
HUZ49_RS17470 (3644486) 3644486..3645919 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
HUZ49_RS17475 (3645976) 3645976..3646719 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T161723 WP_000244772.1 NZ_CP055170:c3641641-3641234 [Shigella flexneri]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T161723 NZ_CP071259:3762314-3762532 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT161723 WP_000354046.1 NZ_CP055170:c3641888-3641622 [Shigella flexneri]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT161723 NZ_CP071259:3761914-3762288 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References