Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 3185743..3186368 Replicon chromosome
Accession NZ_CP055170
Organism Shigella flexneri strain STEFF_22

Toxin (Protein)


Gene name vapC Uniprot ID A0A2J1DF47
Locus tag HUZ49_RS15305 Protein ID WP_000911331.1
Coordinates 3185743..3186141 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag HUZ49_RS15310 Protein ID WP_000450524.1
Coordinates 3186141..3186368 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ49_RS15285 (3181621) 3181621..3181821 + 201 WP_000383836.1 YpfN family protein -
HUZ49_RS15290 (3181931) 3181931..3182629 - 699 WP_000679812.1 esterase -
HUZ49_RS15295 (3182703) 3182703..3184718 - 2016 WP_001543081.1 tRNA cytosine(34) acetyltransferase TmcA -
HUZ49_RS15300 (3184733) 3184733..3185596 - 864 WP_001267507.1 neutral zinc metallopeptidase -
HUZ49_RS15305 (3185743) 3185743..3186141 - 399 WP_000911331.1 type II toxin-antitoxin system VapC family toxin Toxin
HUZ49_RS15310 (3186141) 3186141..3186368 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
HUZ49_RS15315 (3186522) 3186522..3187235 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
HUZ49_RS15320 (3187448) 3187448..3188482 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
HUZ49_RS15325 (3188499) 3188499..3189377 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
HUZ49_RS15330 (3189523) 3189523..3190095 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
HUZ49_RS15335 (3190095) 3190095..3190565 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 3125199..3293710 168511


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14862.19 Da        Isoelectric Point: 9.2216

>T161721 WP_000911331.1 NZ_CP055170:c3186141-3185743 [Shigella flexneri]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLVELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T161721 NZ_CP071259:2840286-2840393 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT161721 WP_000450524.1 NZ_CP055170:c3186368-3186141 [Shigella flexneri]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT161721 NZ_CP071259:c2840236-2840173 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTAAACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J1DF47

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References