Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1112474..1113092 Replicon chromosome
Accession NZ_CP055170
Organism Shigella flexneri strain STEFF_22

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HUZ49_RS05355 Protein ID WP_001291435.1
Coordinates 1112474..1112692 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HUZ49_RS05360 Protein ID WP_000344800.1
Coordinates 1112718..1113092 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ49_RS05320 (1107764) 1107764..1108336 + 573 WP_000779831.1 YbaY family lipoprotein -
HUZ49_RS05325 (1108367) 1108367..1108678 - 312 WP_000409911.1 MGMT family protein -
HUZ49_RS05335 (1109057) 1109057..1109410 + 354 WP_000878140.1 DUF1428 domain-containing protein -
HUZ49_RS05340 (1109452) 1109452..1111002 - 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HUZ49_RS05345 (1111166) 1111166..1111636 - 471 WP_000136192.1 YlaC family protein -
HUZ49_RS05350 (1111752) 1111752..1112303 - 552 WP_000102556.1 maltose O-acetyltransferase -
HUZ49_RS05355 (1112474) 1112474..1112692 - 219 WP_001291435.1 HHA domain-containing protein Toxin
HUZ49_RS05360 (1112718) 1112718..1113092 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HUZ49_RS05365 (1113638) 1113638..1116787 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
HUZ49_RS05370 (1116810) 1116810..1118003 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T161707 WP_001291435.1 NZ_CP055170:c1112692-1112474 [Shigella flexneri]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T161707 NZ_CP071259:379251-379526 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT161707 WP_000344800.1 NZ_CP055170:c1113092-1112718 [Shigella flexneri]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT161707 NZ_CP071259:379523-379882 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References