Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | sanaTA/AbiEii-DUF6088 |
| Location | 450967..452302 | Replicon | chromosome |
| Accession | NZ_CP055170 | ||
| Organism | Shigella flexneri strain STEFF_22 | ||
Toxin (Protein)
| Gene name | sanaT | Uniprot ID | F4VMR9 |
| Locus tag | HUZ49_RS02095 | Protein ID | WP_000342497.1 |
| Coordinates | 451355..452302 (+) | Length | 316 a.a. |
Antitoxin (Protein)
| Gene name | sanaA | Uniprot ID | A0A228Z6F8 |
| Locus tag | HUZ49_RS02090 | Protein ID | WP_000152739.1 |
| Coordinates | 450967..451362 (+) | Length | 132 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HUZ49_RS02075 (447039) | 447039..448205 | - | 1167 | WP_001293040.1 | restriction endonuclease subunit S | - |
| HUZ49_RS02080 (448202) | 448202..449758 | - | 1557 | WP_000202816.1 | type I restriction-modification system subunit M | - |
| HUZ49_RS02085 (450064) | 450064..450897 | + | 834 | WP_001192741.1 | DUF4942 domain-containing protein | - |
| HUZ49_RS02090 (450967) | 450967..451362 | + | 396 | WP_000152739.1 | DUF6088 family protein | Antitoxin |
| HUZ49_RS02095 (451355) | 451355..452302 | + | 948 | WP_000342497.1 | nucleotidyl transferase AbiEii/AbiGii toxin family protein | Toxin |
| HUZ49_RS02100 (452340) | 452340..452525 | - | 186 | WP_000066585.1 | hypothetical protein | - |
| HUZ49_RS02105 (452624) | 452624..452767 | - | 144 | Protein_405 | HNH endonuclease | - |
| HUZ49_RS02110 (452904) | 452904..453554 | + | 651 | WP_001037970.1 | HNH endonuclease | - |
| HUZ49_RS02115 (453562) | 453562..454542 | - | 981 | WP_001366032.1 | sialate O-acetylesterase | - |
| HUZ49_RS02120 (454607) | 454607..455713 | - | 1107 | WP_001044128.1 | N-acetylneuraminate epimerase | - |
| HUZ49_RS02125 (455733) | 455733..456449 | - | 717 | WP_001295734.1 | N-acetylneuraminic acid outer membrane channel NanC | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | - | fimB / fimE | 450064..459581 | 9517 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 316 a.a. Molecular weight: 35588.79 Da Isoelectric Point: 4.9737
>T161705 WP_000342497.1 NZ_CP055170:451355-452302 [Shigella flexneri]
MDDLTTLFSEVADALGIESVAIVEKDHYIVELLQLLKPLVFDTHQLVFAGGTALAKSGVALNRMSEDVDIKLVPKDAFQA
LSRTQRKNTRKEIVQAVSSAIASSGVFNFDDDYPKVTRDEYRYNDIPVRYPQRVVQAPCLRPFIRLELMEAKLLEPSELR
DIHSLVTGITGKAAIVSAFPCATIISTQAEKLVAMLRRTAAVVRDVERFDDASLVRHIYDTNCIVETSHVDMSLLVDFVQ
QTIEQDIQRYGNQYPQFCASPVDELKMGLDELANNPLYQMRYQQFVSPMVYGERKITWDEAYSRFRSLALAILNA
MDDLTTLFSEVADALGIESVAIVEKDHYIVELLQLLKPLVFDTHQLVFAGGTALAKSGVALNRMSEDVDIKLVPKDAFQA
LSRTQRKNTRKEIVQAVSSAIASSGVFNFDDDYPKVTRDEYRYNDIPVRYPQRVVQAPCLRPFIRLELMEAKLLEPSELR
DIHSLVTGITGKAAIVSAFPCATIISTQAEKLVAMLRRTAAVVRDVERFDDASLVRHIYDTNCIVETSHVDMSLLVDFVQ
QTIEQDIQRYGNQYPQFCASPVDELKMGLDELANNPLYQMRYQQFVSPMVYGERKITWDEAYSRFRSLALAILNA
Download Length: 948 bp
>T161705 NZ_CP071256:28880-29296 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTAGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAATAA
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTAGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAATAA
Antitoxin
Download Length: 132 a.a. Molecular weight: 14605.90 Da Isoelectric Point: 11.6915
>AT161705 WP_000152739.1 NZ_CP055170:450967-451362 [Shigella flexneri]
MTIKARIQSRLKRSKRYVFTRDDFKDIAGYDQVGRVLRDLVREGQLLKVGYGVYTKARRNGITGKVMPAAPGGSSAVIVE
TLERLKVRYRFAGATQAYNSGTSTQIPASPEIKTPRRFKRVLSVGNSRLNG
MTIKARIQSRLKRSKRYVFTRDDFKDIAGYDQVGRVLRDLVREGQLLKVGYGVYTKARRNGITGKVMPAAPGGSSAVIVE
TLERLKVRYRFAGATQAYNSGTSTQIPASPEIKTPRRFKRVLSVGNSRLNG
Download Length: 396 bp
>AT161705 NZ_CP071256:28653-28883 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGTGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGTGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A1L5BAQ6 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A228Z6F8 |