Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 400882..401477 Replicon chromosome
Accession NZ_CP055170
Organism Shigella flexneri strain STEFF_22

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag HUZ49_RS01865 Protein ID WP_000239579.1
Coordinates 401127..401477 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID A0A2J1DIF8
Locus tag HUZ49_RS01860 Protein ID WP_001223209.1
Coordinates 400882..401133 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ49_RS01850 (396547) 396547..400326 + 3780 WP_063108276.1 autotransporter assembly complex protein TamB -
HUZ49_RS01855 (400329) 400329..400670 + 342 WP_001219160.1 gamma-glutamylcyclotransferase -
HUZ49_RS01860 (400882) 400882..401133 + 252 WP_001223209.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
HUZ49_RS01865 (401127) 401127..401477 + 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
HUZ49_RS01870 (401557) 401557..402087 - 531 WP_000055075.1 inorganic diphosphatase -
HUZ49_RS01875 (402397) 402397..403353 + 957 WP_000265912.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
HUZ49_RS01880 (403493) 403493..404995 + 1503 WP_000210559.1 sugar ABC transporter ATP-binding protein -
HUZ49_RS01885 (405009) 405009..406031 + 1023 WP_001351397.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T161704 WP_000239579.1 NZ_CP055170:401127-401477 [Shigella flexneri]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T161704 NZ_CP071253:53921-54403 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9301.61 Da        Isoelectric Point: 4.4201

>AT161704 WP_001223209.1 NZ_CP055170:400882-401133 [Shigella flexneri]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWSKSTPAGD
EIW

Download         Length: 252 bp

>AT161704 NZ_CP071253:53667-53933 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A2J1DIF8

References