Detailed information of TA system TA161703

Bioinformatically predicted

Overview

TA module

• Type: V
• Classification (family/domain): ghoTS/ghoT-GhoS
• Location: 297881..298378
• Replicon: chromosome
• Accession: NZ_CP055170
• Organism: Shigella flexneri strain STEFF_22

Toxin (Protein)

• Gene name: ghoT
• Uniprot ID: L4JJG6
• Locus tag: HUZ49_RS01345
• Protein ID: WP_001173343.1
• Coordinates: 298205..298378 (+)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: ghoS
• Uniprot ID: -
• Locus tag: HUZ49_RS01340
• Protein ID: WP_071988580.1
• Coordinates: 297881..298177 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ49_RS01330 (293240)	293240..297169	+	3930	WP_046201569.1	autotransporter outer membrane beta-barrel domain-containing protein	-
HUZ49_RS01335 (297382)	297382..297654	+	273	WP_000405647.1	GNAT family N-acetyltransferase	-
HUZ49_RS01340 (297881)	297881..298177	+	297	WP_071988580.1	type V toxin-antitoxin system endoribonuclease antitoxin GhoS	Antitoxin
HUZ49_RS01345 (298205)	298205..298378	+	174	WP_001173343.1	type V toxin-antitoxin system toxin GhoT	Toxin
HUZ49_RS01350 (298497)	298497..300014	-	1518	WP_001295087.1	lysine--tRNA ligase	-
HUZ49_RS01355 (300251)	300251..301708	-	1458	WP_000856829.1	dipeptide/tripeptide permease DtpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	272669..307232	34563

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin GhoT/OrtT (4-57)

Antitoxin

• Endoribonuclease antitoxin GhoS (5-94)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6555.06 Da
• Isoelectric Point: 10.2288

>T161703 WP_001173343.1 NZ_CP055170:298205-298378 [Shigella flexneri]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Nucleotide

• Length: 174 bp

>T161703 NZ_CP071252:c5294942-5294559 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11482.00 Da
• Isoelectric Point: 4.1705

>AT161703 WP_071988580.1 NZ_CP055170:297881-298177 [Shigella flexneri]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSTIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Nucleotide

• Length: 297 bp

>AT161703 NZ_CP071252:c5295184-5294942 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A0A7U9QI41