Detailed information of TA system TA161699

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 68936..69561
• Replicon: plasmid unnamed1
• Accession: NZ_CP055168
• Organism: Shigella flexneri strain STEFF_24

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HUZ50_RS23925
• Protein ID: WP_000911313.1
• Coordinates: 69163..69561 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: HUZ50_RS23920
• Protein ID: WP_000450520.1
• Coordinates: 68936..69163 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ50_RS23920 (68936)	68936..69163	+	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin
HUZ50_RS23925 (69163)	69163..69561	+	399	WP_000911313.1	type II toxin-antitoxin system VapC family toxin	Toxin
HUZ50_RS23930 (69570)	69570..71723	-	2154	WP_000009324.1	type IV conjugative transfer system coupling protein TraD	-
HUZ50_RS23935 (71976)	71976..72707	-	732	WP_023565183.1	conjugal transfer complement resistance protein TraT	-
HUZ50_RS23940 (72739)	72739..73236	-	498	WP_000605857.1	entry exclusion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / dfrA14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaCTX-M-15 / sitABCD	iutA / iucD / iucC / iucB / iucA	1..125110	125110

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14901.15 Da
• Isoelectric Point: 7.8605

>T161699 WP_000911313.1 NZ_CP055168:69163-69561 [Shigella flexneri]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T161699 NZ_CP071252:4075641-4075859 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT161699 WP_000450520.1 NZ_CP055168:68936-69163 [Shigella flexneri]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT161699 NZ_CP071252:4075240-4075614 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Antitoxin

• AlphaFold DB: V0VCB2