Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4420419..4420916 Replicon chromosome
Accession NZ_CP055167
Organism Shigella flexneri strain STEFF_24

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HUZ50_RS21325 Protein ID WP_001173343.1
Coordinates 4420419..4420592 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag HUZ50_RS21330 Protein ID WP_000398619.1
Coordinates 4420620..4420916 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ50_RS21315 (4417089) 4417089..4418546 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
HUZ50_RS21320 (4418783) 4418783..4420300 + 1518 WP_001295074.1 lysine--tRNA ligase -
HUZ50_RS21325 (4420419) 4420419..4420592 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HUZ50_RS21330 (4420620) 4420620..4420916 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HUZ50_RS21335 (4421143) 4421143..4421415 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
HUZ50_RS21340 (4421427) 4421427..4421657 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
HUZ50_RS21345 (4421838) 4421838..4423469 + 1632 WP_001216487.1 sensor histidine kinase -
HUZ50_RS21350 (4423466) 4423466..4424185 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T161692 WP_001173343.1 NZ_CP055167:c4420592-4420419 [Shigella flexneri]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T161692 NZ_CP071248:2105433-2105756 [Bifidobacterium longum subsp. infantis]
ATGAAACGAGGTGAGATTTGGACGTTGCAGGCCGATGGCTACGCCAGCAAGCCACAGCCGGTTGTCATCGTGCAGAGCGA
TGCCGTCGATCGGTTCGATTCCGTCATCACTTGTCTGCTGACCTCATACGATTCCTCCGACATCGACACGCGGGTCCGAT
TGGAACCAAGTCCGGAGAACGGCCTGAACAAAGTGAGTTACGTGATGACGGACAAGATCGTCACCGTCGACCGCAAACTG
CTGGGATACCAAGTCGGCGTGGTTGATGATGCAGCCATGGCGAATATCGGCCGTCAGCTCATGCGGGTTCTAGGTTTGCT
GTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT161692 WP_000398619.1 NZ_CP055167:c4420916-4420620 [Shigella flexneri]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT161692 NZ_CP071248:2105201-2105446 [Bifidobacterium longum subsp. infantis]
ATGAGCATACAGATTGCCACGCGAGTTAGTGACGAGCAGGCTGCGTTGTTTAAGGAGACGACCCGACAGCTGGGTACCAC
CCCGGCCGATGCCCTGCGTATGTTCATCAGCGCGTTCAATGATTACCGTGGGTTCCCCTATGAGGTGCGTTTGCCACGAA
ATGACGTGGAGCCGTTTGCGTCCGAGCGGGACGCTACTGAGTATGCCAGTCGTCTCGCCTTGAGGATGTCCGATGAAACG
AGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References