Detailed information of TA system TA161691

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4323726..4324321
• Replicon: chromosome
• Accession: NZ_CP055167
• Organism: Shigella flexneri strain STEFF_24

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: HUZ50_RS20835
• Protein ID: WP_000239581.1
• Coordinates: 4323726..4324076 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: HUZ50_RS20840
• Protein ID: WP_001223213.1
• Coordinates: 4324070..4324321 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ50_RS20815 (4319180)	4319180..4320202	-	1023	WP_063625321.1	ABC transporter permease	-
HUZ50_RS20820 (4320216)	4320216..4321718	-	1503	WP_063625322.1	sugar ABC transporter ATP-binding protein	-
HUZ50_RS20825 (4321851)	4321851..4322807	-	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
HUZ50_RS20830 (4323117)	4323117..4323647	+	531	WP_000055075.1	inorganic diphosphatase	-
HUZ50_RS20835 (4323726)	4323726..4324076	-	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
HUZ50_RS20840 (4324070)	4324070..4324321	-	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
HUZ50_RS20845 (4324533)	4324533..4324874	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
HUZ50_RS20850 (4324877)	4324877..4328656	-	3780	WP_063625323.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T161691 WP_000239581.1 NZ_CP055167:c4324076-4323726 [Shigella flexneri]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T161691 NZ_CP071248:2053387-2053743 [Bifidobacterium longum subsp. infantis]
ATGATGAAGACTGATCCGCGCCAGTTCGAGATATGGTGGGTGCCGTTCGCTTTTCCCGACAAGCCAGGCAAAGCGAAGAA
CCGTCCCAGCGTGATCCTCCAATGGGACGACGGCACCCGCATCGCCTTGGTGACCAAGGTCACCGGCAACACCTGGCGCG
ACGAACCGGGCTATGTGGTGCTGCGTGACTGGCAGGACGCGGGACTGAGCAAGCCCAGCGCCGTACGATGCTCGCAACTG
CTGCGGCTGCCAAGCAATCTTTCCCTCGACGACGGCCCGACAGGAAGACTTTCGGCATATGATGCCGGCAGCGTCGCCTG
GGAGATCAACGAACTCTATCCGGGCCTACTAGCCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT161691 WP_001223213.1 NZ_CP055167:c4324321-4324070 [Shigella flexneri]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT161691 NZ_CP071248:2053107-2053400 [Bifidobacterium longum subsp. infantis]
ATGGCTATCAAGGAGAAGGCCACGATCAGTCTGGATGCGCAGACCAAGCGTGACGGCATCGCCATACTTGACGCCATGGG
GTTGAACCTGAGCACGTTTGCCGAAATGTCTCTCAGACAATTGGTACGCGACGGGCGTCTGCCATTCACGCCAAGCGTCC
GCCCTTCATTCGAAAAAGACAACGAGGGATATCCCCTGTTCAAAGCGAACATGGATGATCCCCGCATCGTGACGCCCCAG
ATACGTGATGGTGCCGTGATCCTGCCGGAGGGTTGGGACGATGATGAAGACTGA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7