Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3586865..3587702 Replicon chromosome
Accession NZ_CP055167
Organism Shigella flexneri strain STEFF_24

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag HUZ50_RS17440 Protein ID WP_000227784.1
Coordinates 3587160..3587702 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag HUZ50_RS17435 Protein ID WP_001297137.1
Coordinates 3586865..3587176 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ50_RS17410 (3581885) 3581885..3582832 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
HUZ50_RS17415 (3582854) 3582854..3584845 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
HUZ50_RS17420 (3584835) 3584835..3585449 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
HUZ50_RS17425 (3585449) 3585449..3585778 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
HUZ50_RS17430 (3585790) 3585790..3586680 + 891 WP_000971336.1 heme o synthase -
HUZ50_RS17435 (3586865) 3586865..3587176 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
HUZ50_RS17440 (3587160) 3587160..3587702 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
HUZ50_RS17445 (3587758) 3587758..3588693 - 936 WP_001297127.1 tetratricopeptide repeat protein -
HUZ50_RS17450 (3589101) 3589101..3590465 + 1365 WP_001000974.1 MFS transporter -
HUZ50_RS17455 (3590593) 3590593..3591084 - 492 WP_001138904.1 nucleotide binding protein YajQ -
HUZ50_RS17460 (3591252) 3591252..3592163 + 912 WP_000705874.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T161687 WP_000227784.1 NZ_CP055167:3587160-3587702 [Shigella flexneri]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T161687 NZ_CP071248:c1585852-1585568 [Bifidobacterium longum subsp. infantis]
ATGAGCACCGAATTCACCGACTACGACACCAGCGAACACCTCGAAAACGAACAGGACATCATCGCCTACCTCAACGCCAT
AGCCGAATACGACGACCCCGCACTCATGCAGGCCGCGCTCGGCAACGTCGCCAAGGCCCGGGGCATGACCCAAATCGCCA
AGGACGCGGGCGTGGGGCGCGAAAGCCTCTACAAGAGCCTCAGCAAGGACGGCAACCCCAGCTTCCAAACCATCGCCAAG
GTAATCCACGCCCTCGGCGGACGCCTCACCATCCAAGCCGCCTGA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT161687 WP_001297137.1 NZ_CP055167:3586865-3587176 [Shigella flexneri]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT161687 NZ_CP071248:c1586151-1585849 [Bifidobacterium longum subsp. infantis]
ATCAAGCAATCCGCCGAATACCGCAAGTGGTTCAAGAAACTCAGGGACCACAAAGCGAAGGCCGCCATCCAAGCCCGGCT
CGATGCCTGCAAGCTCGCAGGCAGGCCATTCGGCGACATCAAACCCGTGGGAGGCCCGGTCAGCGAGATGCGGTTCCACA
CCGGGGCCGGATACCGCGTCTACTTCGCCATGCAGGGCAACGTGCTCATGCTGCTGCTCGCAGGCGGCGACAAAAGCACC
CAGCAGACCGACATCAGACAAGCCCACGACATCCTCAACGACTACAAGGAACAACGACAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References