Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 911557..912211 Replicon chromosome
Accession NZ_CP055167
Organism Shigella flexneri strain STEFF_24

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag HUZ50_RS04435 Protein ID WP_000244772.1
Coordinates 911804..912211 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HUZ50_RS04430 Protein ID WP_000354046.1
Coordinates 911557..911823 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ50_RS04405 (906845) 906845..907588 + 744 WP_000951964.1 SDR family oxidoreductase -
HUZ50_RS04410 (907645) 907645..909078 - 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
HUZ50_RS04415 (909123) 909123..909434 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
HUZ50_RS04420 (909598) 909598..910257 + 660 WP_000250274.1 hemolysin III family protein -
HUZ50_RS04425 (910334) 910334..911314 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
HUZ50_RS04430 (911557) 911557..911823 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HUZ50_RS04435 (911804) 911804..912211 + 408 WP_000244772.1 protein YgfX Toxin
HUZ50_RS04440 (912251) 912251..912772 - 522 WP_063625410.1 flavodoxin FldB -
HUZ50_RS04445 (912884) 912884..913780 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HUZ50_RS04450 (913805) 913805..914515 + 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HUZ50_RS04455 (914521) 914521..916254 + 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T161673 WP_000244772.1 NZ_CP055167:911804-912211 [Shigella flexneri]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T161673 NZ_CP071244:c3109565-3109167 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGTTCAGTCATATGCTGGACACCAACATTGTTATCTATGTCATCAAGCGCCGGCCGCTGGAAGTGCTGGAGGCATTCAA
TCGCTATGCAGGTAAAATGGTCATTTCGTCGATCACTTATGGCGAATTGGTTCACGGGGTGGAGAAAAGCGCTCGTCCAG
CGGTGAACGCCAGAGTCGTTGAGGATTTTGTCTCCCGATTGGACATTCTCGATTACAGCGCCAAAGCGGCATCGCACTAC
GGCAATATCCGGGCTGCGCTGGAACGACAGGGAACGCCTATTGGCGTAAACGATCTACATATTGCCGGGCATGCGCGCAG
CGAAGGATTGATCCTGGTGACCAATAATCGACGCGAATTTGAGCGCGTTGAGGGTTTGCGGCTGGAAAATTGGTTGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT161673 WP_000354046.1 NZ_CP055167:911557-911823 [Shigella flexneri]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT161673 NZ_CP071244:c3109798-3109565 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGACGTTAGGTTCTGTCTTTACTAATAATCGGACACAGGCTGTACGCCTGCCTGCAGAGGCGCGTTTCCCTGATACCGT
TTCTAAAGTGACCGTTCGCGTAGTGGGTAAAGAGCGCATTCTTGCCCCCCTGGAGAATACCTGGGACAGTTTCTTCTGTG
CGGAAAATGAGGTGAGCGATGATTTTCTCAATGAACGTGCCGGACAGCACCAGCGCGAGCGGGAAGCGTTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References