Detailed information of TA system TA161671

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 324690..325490
• Replicon: chromosome
• Accession: NZ_CP055167
• Organism: Shigella flexneri strain STEFF_24

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: HUZ50_RS01480
• Protein ID: WP_000342451.1
• Coordinates: 324963..325490 (+)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: F4NNI1
• Locus tag: HUZ50_RS01475
• Protein ID: WP_001277108.1
• Coordinates: 324690..324956 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ50_RS01455 (320348)	320348..321016	+	669	WP_000617723.1	cell division ATP-binding protein FtsE	-
HUZ50_RS01460 (321009)	321009..322067	+	1059	WP_001042003.1	permease-like cell division protein FtsX	-
HUZ50_RS01465 (322311)	322311..323165	+	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
HUZ50_RS01470 (323437)	323437..324540	+	1104	WP_078167906.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
HUZ50_RS01475 (324690)	324690..324956	+	267	WP_001277108.1	type II toxin-antitoxin system antitoxin AtaR	Antitoxin
HUZ50_RS01480 (324963)	324963..325490	+	528	WP_000342451.1	type II toxin-antitoxin system toxin acetyltransferase AtaT	Toxin
HUZ50_RS01485 (325487)	325487..325870	-	384	WP_000778795.1	aspartate 1-decarboxylase autocleavage activator PanM	-
HUZ50_RS01490 (326294)	326294..327403	+	1110	WP_000827696.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
HUZ50_RS01495 (327451)	327451..328377	+	927	WP_000003004.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
HUZ50_RS01500 (328374)	328374..329651	+	1278	WP_000803798.1	branched chain amino acid ABC transporter permease LivM	-
HUZ50_RS01505 (329648)	329648..330415	+	768	WP_000082110.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19749.73 Da
• Isoelectric Point: 7.3232

>T161671 WP_000342451.1 NZ_CP055167:324963-325490 [Shigella flexneri]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAELPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Nucleotide

• Length: 528 bp

>T161671 NZ_CP071244:1601798-1602106 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGCAATTTACCGTATATGACAATACGTATCCGGCATCAGCCTATCCCTACTTACTGGACGTTCAAAGCGATCTTATTGA
TGTCCTCTCGACCCGGTTAATGATCCCTCTTTATGCTTTGGATAATGTCAGGGTTAAAATTTCTGCACGTTTATGCCCGG
AGATTGAGGTCAACGGCGAAAAGTTTCTTGTTATGACGCATGAGATGGCTGCCGTACGTATTTCGCAGATTGGGAAGGCC
GTTGGTAATGTCAATGAGCACAGGAATCAAATTAAAGCCGCCATCGATTTTCTGATTGATGGTTTTTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9910.31 Da
• Isoelectric Point: 10.0863

>AT161671 WP_001277108.1 NZ_CP055167:324690-324956 [Shigella flexneri]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Nucleotide

• Length: 267 bp

>AT161671 NZ_CP071244:1601559-1601795 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGGCAAATAACAATGTGACGAAAAAGACAGTCAACGTAACCATCGATCGCGATCTTTTCCAGCGCGCAAAATCCCTGGG
CGTCAATGTGTCATCTGTATTGACGGACGCATTGAGCGTCCGTGTCAGGGACATTGAGATTCAACAGTGGCGCGAGCAAA
ATCGTGCGGCGTTAGAAGAACTTAATCGTATCACTGAAGAAAATGGTTTGTTGTCTGATGAATATCGGACATTTTGA

Structures

Antitoxin

• PDB: 6GTS
• PDB: 6AJN
• PDB: 6GTQ
• PDB: 6GTO
• PDB: 6GTR
• PDB: 6AJM
• AlphaFold DB: A0A829CN24