Detailed information of TA system TA161670

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 99102..99754
• Replicon: plasmid unnamed1
• Accession: NZ_CP055163
• Organism: Klebsiella pneumoniae strain STEFF_9

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: HUZ51_RS26160
• Protein ID: WP_239637218.1
• Coordinates: 99102..99527 (-)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A853H7M9
• Locus tag: HUZ51_RS26165
• Protein ID: WP_001261275.1
• Coordinates: 99524..99754 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ51_RS26125 (HUZ51_26075)	94811..94927	+	117	Protein_96	transposase	-
HUZ51_RS26130 (HUZ51_26080)	95052..95222	-	171	Protein_97	LysR family transcriptional regulator	-
HUZ51_RS26135	95233..95460	+	228	Protein_98	IS3 family transposase	-
HUZ51_RS26140 (HUZ51_26090)	95552..97108	-	1557	WP_025999314.1	sensor domain-containing diguanylate cyclase	-
HUZ51_RS26145	97295..97468	-	174	Protein_100	nuclease	-
HUZ51_RS26150 (HUZ51_26095)	97521..98057	-	537	Protein_101	integrase core domain-containing protein	-
HUZ51_RS26155 (HUZ51_26100)	98116..99084	-	969	WP_077254762.1	IS5 family transposase	-
HUZ51_RS26160 (HUZ51_26105)	99102..99527	-	426	WP_239637218.1	type II toxin-antitoxin system VapC family toxin	Toxin
HUZ51_RS26165 (HUZ51_26110)	99524..99754	-	231	WP_001261275.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
HUZ51_RS26170 (HUZ51_26115)	100007..102583	-	2577	WP_017901322.1	nuclease domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	aph(3')-Ia / mph(A) / sul1 / qacE / aadA16 / dfrA27 / ARR-3 / aac(6')-Ib-cr / tet(D) / floR / blaDHA-1 / qnrB4 / catB3 / blaOXA-1	-	1..260949	260949
	-	flank	IS/Tn	-	-	98116..99084	968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 15426.94 Da
• Isoelectric Point: 7.1364

>T161670 WP_239637218.1 NZ_CP055163:c99527-99102 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWIGFVE

Nucleotide

• Length: 426 bp

>T161670 NZ_CP071244:c1108032-1107829 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGACTAAAACCGACTATCTGATGCGTTTACGTAAATGCACCACGATTGATACTCTCGAACGTGTTATTGAGAAAAATAA
GTACGAGCTTTCCGATGATGAACTGGAGCTGTTTTACTCAGCGGCCGATCACCGTCTGGCAGAGCTCACCATGAACAAAC
TGTATGACAAAATCCCCACCTCCGTTTGGAAATATGTCAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8707.72 Da
• Isoelectric Point: 4.3410

>AT161670 WP_001261275.1 NZ_CP055163:c99754-99524 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFDP

Nucleotide

• Length: 231 bp

>AT161670 NZ_CP071244:c1108450-1108082 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGGATGAGTATTCGCCTAAGCGGCACGACATTGCTCAGCTCAAATTCTTGTGCGAAAATTTGTATGATGAAGGTATCGC
TACCCTGGGAGACAGTCATCATGGGTGGGTCAACGATCCGACGTCCTCCGTCAACCTGCAGCTTAATGAGTTGATCGAAC
ATATCGCTTCGTTTGTGATGAGTTATAAAATTAAATACATGGACGAATCTGACCTGTCGGAGTTAGTCGAAGAATATCTC
GACGATACCTATACGCTGTTCAGCAGCTATGGTATCAATGATTCCGACCTGCGCCGCTGGCAAAAAACCAAGGCGAGATT
ATTCAGAATGTTCTCAGGAGAGGACATCTGTACGACAATGAAAACTTAG

Structures

Antitoxin

• AlphaFold DB: A0A853H7M9