Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5197611..5198236 Replicon chromosome
Accession NZ_CP055162
Organism Klebsiella pneumoniae strain STEFF_9

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HUZ51_RS25280 Protein ID WP_002882817.1
Coordinates 5197611..5197994 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HUZ51_RS25285 Protein ID WP_004150355.1
Coordinates 5197994..5198236 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ51_RS25265 (5194977) 5194977..5195879 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HUZ51_RS25270 (5195876) 5195876..5196511 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HUZ51_RS25275 (5196508) 5196508..5197437 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HUZ51_RS25280 (5197611) 5197611..5197994 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HUZ51_RS25285 (5197994) 5197994..5198236 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
HUZ51_RS25290 (5198441) 5198441..5199358 + 918 WP_004181612.1 alpha/beta hydrolase -
HUZ51_RS25295 (5199372) 5199372..5200313 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HUZ51_RS25300 (5200358) 5200358..5200795 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
HUZ51_RS25305 (5200792) 5200792..5201652 - 861 WP_004146232.1 virulence factor BrkB family protein -
HUZ51_RS25310 (5201646) 5201646..5202245 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T161668 WP_002882817.1 NZ_CP055162:c5197994-5197611 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T161668 NZ_CP071242:4535861-4536172 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGCATATCATTTCCAGGGAACCGTTCAATAACGCAACGAAGCAATATCCCAATGAAGCCACTGCTCTCGAGGCCATTTA
TAAAGCATTGCGACGGGGCGAGTTCCGCACGCCGGACGAGTTAAAAGCGCTTTTTCCTTCGCTCGATCGTATGAAATACA
AAGAGAAATGGTGGGTCATTGACGTCGGCGGCAACCACCTGCGGATCTTGTTCTTTGCCAGCTTCGAGACACAGAAAGTC
TTCATAAAGCATATCGTTAGCCACGCCGAATACGACAAGCTGATGCAGCACTATCGAAGGAGTTCATCATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT161668 WP_004150355.1 NZ_CP055162:c5198236-5197994 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT161668 NZ_CP071242:4536169-4536591 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGATTACAGATGCCATTAAGGCGGCCGATGCGCTACTGCGCGCCGTGCCCTTGTTGCAGGGCAGCCGCGCTCAGCAAGA
CTACCGGGAAGCGCTTGAATTGGTGGAATACCTGCTGGAAAACGACGAACACCATCCGCTGATTGACATGCTGGCAAAAA
AGATCGCCGAATATGAAGACAACAGCGCCGAATTTACCGCATTCAACCAGCGTATCGCCCAGTTACCACAGGGGGTAGCC
GCGCTTCGCGTCCTTATGGAACAGCACCAACTCAGCCAAAGTGATTTTGAACATGAGATCGGTAAAAAGTCGTTGGTTAA
CCAAATCCTGAACGGTAAAAGATCGCTGACCATTCCGCACATTATGGCGCTGGCCAAGCGTTTCAATCTGCCCCCGGCCC
TGTTCCTGCCGGAAGCGGATTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References