Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4617466..4618276 Replicon chromosome
Accession NZ_CP055162
Organism Klebsiella pneumoniae strain STEFF_9

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag HUZ51_RS22600 Protein ID WP_004178461.1
Coordinates 4617466..4617999 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag HUZ51_RS22605 Protein ID WP_002887278.1
Coordinates 4618010..4618276 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ51_RS22595 (4616297) 4616297..4617418 + 1122 WP_002887282.1 cupin domain-containing protein -
HUZ51_RS22600 (4617466) 4617466..4617999 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
HUZ51_RS22605 (4618010) 4618010..4618276 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
HUZ51_RS22610 (4618379) 4618379..4619812 - 1434 WP_004178459.1 Cu(+)/Ag(+) sensor histidine kinase -
HUZ51_RS22615 (4619802) 4619802..4620485 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
HUZ51_RS22620 (4620657) 4620657..4622042 + 1386 WP_004178457.1 efflux transporter outer membrane subunit -
HUZ51_RS22625 (4622060) 4622060..4622404 + 345 WP_004197273.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T161665 WP_004178461.1 NZ_CP055162:c4617999-4617466 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T161665 NZ_CP071242:c3787969-3787580 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGTACATGTTTGATACCAACACCGTCAGCCACTTGTTTCGGCAGCATCCGCAGGTGCTTAACGTCATGGAAAAGCTCCC
GCCTTCTGCCGTATGTATTTCCAGCGTCACGGAGGCGGAACTGCTTTACGGTGTGGCTAAACGGCGGAACAAGGCGTTGC
AAAGCATGGTCGAGGCCTTTCTTGCTGCAGTGACGGTCTATGCATGGGATAGCGAGGCCGCCCGTTGCTACGGTGAAATG
CGGGCAACCATGGAGAGGAAGGGCAAAATCATGGGCGCGATCGATCAACTGATCGCCGCTCACGCGCAGAGCCGTGGCGC
GACGCTGGTGACCAACGATCGCGCATTCGCCATGGTGCCCGGGTTGGCGGTTGAAGACTGGACGCGCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT161665 WP_002887278.1 NZ_CP055162:c4618276-4618010 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT161665 NZ_CP071242:c3788236-3787973 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGGAAAGAATCGCAAAGCTGTTTAAAAACGGCAGAAATCAGGCGGTGAGGTTACCGGTGGAATTTGAATTTGATACGGA
TCGTGTCTATATCCGTCGCGATCGGGAGGGCAACGTGATTTTATCCAAATATCCGGCAAAGCCGGACAGCTGGGCACCGC
TGCTGAGCATTATCGCCCAAACGCCGGTGCCGCAAGAATTTCTTGGCGCGGAAGATCGTCAGCAAGGGATGGCAGACCGC
GATCCGTTCTCAGAGGAAGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References