Detailed information of TA system TA161632

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 3913877..3914794
• Replicon: chromosome
• Accession: NZ_CP055160
• Organism: Bacillus velezensis strain WLYS23

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: HUW56_RS19270
• Protein ID: WP_007407256.1
• Coordinates: 3914048..3914794 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: HUW56_RS19265
• Protein ID: WP_003154807.1
• Coordinates: 3913877..3914047 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUW56_RS19230	3909110..3910732	+	1623	WP_032874601.1	pyocin knob domain-containing protein	-
HUW56_RS19235	3910745..3911116	+	372	WP_032874603.1	YomQ/XkdW protein, phage-like element PBSX	-
HUW56_RS19240	3911121..3911318	+	198	WP_032874605.1	XkdX family protein	-
HUW56_RS19245	3911375..3912136	+	762	WP_032874607.1	hypothetical protein	-
HUW56_RS19250	3912188..3912451	+	264	WP_003154815.1	hemolysin XhlA family protein	-
HUW56_RS19255	3912465..3912728	+	264	WP_003154813.1	phage holin	-
HUW56_RS19260	3912742..3913620	+	879	WP_032874609.1	N-acetylmuramoyl-L-alanine amidase	-
HUW56_RS19265	3913877..3914047	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
HUW56_RS19270	3914048..3914794	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
HUW56_RS19275	3914899..3915897	-	999	WP_032874611.1	inorganic phosphate transporter	-
HUW56_RS19280	3915910..3916527	-	618	WP_032874614.1	DUF47 domain-containing protein	-
HUW56_RS19285	3916813..3918129	-	1317	WP_032874616.1	amino acid permease	-
HUW56_RS19290	3918451..3919401	+	951	WP_032874618.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T161632 WP_007407256.1 NZ_CP055160:c3914794-3914048 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T161632 NZ_CP071234:4535883-4536194 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGCATATCATTTCCAGGGAACCGTTCAATAACGCAACGAAGCAATATCCCAATGAAGCCACTGCTCTCGAGGCCATTTA
TAAAGCATTGCGACGGGGCGAGTTCCGCACGCCGGACGAGTTAAAAGCGCTTTTTCCTTCGCTCGATCGTATGAAATACA
AAGAGAAATGGTGGGTCATTGACGTCGGCGGCAACCACCTGCGGATCTTGTTCTTTGCCAGCTTCGAGACACAGAAAGTC
TTCATAAAGCATATCGTTAGCCACGCCGAATACGACAAGCTGATGCAGCACTATCGAAGGAGTTCATCATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT161632 WP_003154807.1 NZ_CP055160:c3914047-3913877 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT161632 NZ_CP071234:4536191-4536613 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGATTACAGATGCCATTAAGGCGGCCGATGCGCTACTGCGCGCCGTGCCCTTGTTGCAGGGCAGCCGCGCTCAGCAAGA
CTACCGGGAAGCGCTTGAATTGGTGGAATACCTGCTGGAAAACGACGAACACCATCCGCTGATTGACATGCTGGCAAAAA
AGATCGCCGAATATGAAGACAACAGCGCCGAATTTACCGCATTCAACCAGCGTATCGCCCAGTTACCACAGGGGGTAGCC
GCGCTTCGCGTCCTTATGGAACAGCACCAACTCAGCCAAAGTGATTTTGAACATGAGATCGGTAAAAAGTCGTTGGTTAA
CCAAATCCTGAACGGTAAAAGATCGCTGACCATTCCGCACATTATGGCGCTGGCCAAGCGTTTCAATCTGCCCCCGGCCC
TGTTCCTGCCGGAAGCGGATTAG

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14