Detailed information of TA system TA161631

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3162654..3163291
• Replicon: chromosome
• Accession: NZ_CP055160
• Organism: Bacillus velezensis strain WLYS23

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: HUW56_RS15320
• Protein ID: WP_003156187.1
• Coordinates: 3162941..3163291 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: HUW56_RS15315
• Protein ID: WP_003156188.1
• Coordinates: 3162654..3162935 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUW56_RS15295	3159019..3159618	-	600	WP_032872997.1	rhomboid family intramembrane serine protease	-
HUW56_RS15300	3159711..3160076	+	366	WP_007609580.1	holo-ACP synthase	-
HUW56_RS15305	3160241..3161248	+	1008	WP_032872999.1	outer membrane lipoprotein carrier protein LolA	-
HUW56_RS15310	3161365..3162534	+	1170	WP_032873001.1	alanine racemase	-
HUW56_RS15315	3162654..3162935	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
HUW56_RS15320	3162941..3163291	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
HUW56_RS15325	3163409..3164230	+	822	WP_003156182.1	STAS domain-containing protein	-
HUW56_RS15330	3164235..3164600	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
HUW56_RS15335	3164603..3165004	+	402	WP_003156178.1	anti-sigma regulatory factor	-
HUW56_RS15340	3165016..3166023	+	1008	WP_007609589.1	PP2C family protein-serine/threonine phosphatase	-
HUW56_RS15345	3166087..3166416	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
HUW56_RS15350	3166413..3166895	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
HUW56_RS15355	3166861..3167649	+	789	WP_032873003.1	RNA polymerase sigma factor SigB	-
HUW56_RS15360	3167649..3168251	+	603	WP_007410234.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T161631 WP_003156187.1 NZ_CP055160:3162941-3163291 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T161631 NZ_CP071234:c4455866-4455612 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGAACAAACGACACCAAAAAACGCTGTCAGATGTGTTTGCCCGGCCTGTCAACGGTTCTATAAAGTGGTCTGATATTGA
GGCGCTTTTTACCGCATTAGGGGCGGAGATTCACGAAAGGGAAGGTTCTAGGATCGCGGTGCTGTTGAAAGGTGAAAAAA
GAGTCTTTCACCGGCCACACCCCAGACCTACCACTGACAAGGGGGCGGTTAACTCCATTCGGATCTGGTTGGATAGCTTA
GGAATAAAACCATGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT161631 WP_003156188.1 NZ_CP055160:3162654-3162935 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT161631 NZ_CP071234:c4455612-4455271 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGAATAACACACTGAAAATTGACGGCCATACGGCCGTCATCAACTTCGACCCTGAAATTGAAATGTTCCGGGGCGAGTT
TGTCGGGCTGAATGGCGGCGCCGACTTCTACGCCTACAGCGTGGACGAGCTGAAGAAAGAAGGCGCGATCTCACTCGCGG
TCTTTCTCGATGAGTGCCATAAAGACGGCATCGAGCCTTACAAGTCGTACAGCGGCAAAGTAACCACCCGCCTGTCGCCG
GAACGCCATCAGGCGTTAGCCATTGCCGCACAGGCCACCGGGCAGTCGATTAATGAACTGCTGAATGAAGGTGTTGATCT
GGTTATCGAAAAGCATTCCTGA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5