Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5045039..5045664 Replicon chromosome
Accession NZ_CP055119
Organism Klebsiella pneumoniae strain SWHIN_100

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HUZ80_RS24520 Protein ID WP_002882817.1
Coordinates 5045039..5045422 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HUZ80_RS24525 Protein ID WP_004150355.1
Coordinates 5045422..5045664 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ80_RS24505 (5042405) 5042405..5043307 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HUZ80_RS24510 (5043304) 5043304..5043939 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HUZ80_RS24515 (5043936) 5043936..5044865 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HUZ80_RS24520 (5045039) 5045039..5045422 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HUZ80_RS24525 (5045422) 5045422..5045664 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
HUZ80_RS24530 (5045869) 5045869..5046786 + 918 WP_065803683.1 alpha/beta hydrolase -
HUZ80_RS24535 (5046800) 5046800..5047741 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HUZ80_RS24540 (5047786) 5047786..5048223 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
HUZ80_RS24545 (5048220) 5048220..5049080 - 861 WP_004146232.1 virulence factor BrkB family protein -
HUZ80_RS24550 (5049074) 5049074..5049673 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T161626 WP_002882817.1 NZ_CP055119:c5045422-5045039 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T161626 NZ_CP071234:1603058-1603366 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGCAATTTACCGTATATGACAATACGTATCCGGCATCAGCCTATCCCTACTTACTGGACGTTCAAAGCGATCTTATTGA
TGTCCTCTCGACCCGGTTAATGATCCCTCTTTATGCTTTGGATAATGTCAGGGTTAAAATTTCTGCACGTTTATGCCCGG
AGATTGAGGTCAACGGCGAAAAGTTTCTTGTTATGACGCATGAGATGGCTGCCGTACGTATTTCGCAGATTGGGAAGGCC
GTTGGTAATGTCAATGAGCACAGGAATCAAATTAAAGCCGCCATCGATTTTCTGATTGATGGTTTTTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT161626 WP_004150355.1 NZ_CP055119:c5045664-5045422 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT161626 NZ_CP071234:1602819-1603055 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGGCAAATAACAATGTGACGAAAAAGACAGTCAACGTAACCATCGATCGCGATCTTTTCCAGCGCGCAAAATCCCTGGG
CGTCAATGTGTCATCTGTATTGACGGACGCATTGAGCGTCCGTGTCAGGGACATTGAGATTCAACAGTGGCGCGAGCAAA
ATCGTGCGGCGTTAGAAGAACTTAATCGTATCACTGAAGAAAATGGTTTGTTGTCTGATGAATATCGGACATTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References