Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4451204..4452014 Replicon chromosome
Accession NZ_CP055119
Organism Klebsiella pneumoniae strain SWHIN_100

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag HUZ80_RS21730 Protein ID WP_004178461.1
Coordinates 4451204..4451737 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag HUZ80_RS21735 Protein ID WP_002887278.1
Coordinates 4451748..4452014 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ80_RS21725 (4450035) 4450035..4451156 + 1122 WP_004214138.1 cupin domain-containing protein -
HUZ80_RS21730 (4451204) 4451204..4451737 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
HUZ80_RS21735 (4451748) 4451748..4452014 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
HUZ80_RS21740 (4452117) 4452117..4453550 - 1434 WP_023283180.1 Cu(+)/Ag(+) sensor histidine kinase -
HUZ80_RS21745 (4453540) 4453540..4454223 - 684 WP_004214143.1 copper response regulator transcription factor CusR -
HUZ80_RS21750 (4454395) 4454395..4455780 + 1386 WP_023283179.1 efflux transporter outer membrane subunit -
HUZ80_RS21755 (4455798) 4455798..4456142 + 345 WP_021462623.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T161623 WP_004178461.1 NZ_CP055119:c4451737-4451204 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T161623 NZ_CP071232:4534604-4534915 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGCATATCATTTCCAGGGAACCGTTCAATAACGCAACGAAGCAATATCCCAATGAAGCCACTGCTCTCGAGGCCATTTA
TAAAGCATTGCGACGGGGCGAGTTCCGCACGCCGGACGAGTTAAAAGCGCTTTTTCCTTCGCTCGATCGTATGAAATACA
AAGAGAAATGGTGGGTCATTGACGTCGGCGGCAACCACCTGCGGATCTTGTTCTTTGCCAGCTTCGAGACACAGAAAGTC
TTCATAAAGCATATCGTTAGCCACGCCGAATACGACAAGCTGATGCAGCACTATCGAAGGAGTTCATCATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT161623 WP_002887278.1 NZ_CP055119:c4452014-4451748 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT161623 NZ_CP071232:4534912-4535334 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGATTACAGATGCCATTAAGGCGGCCGATGCGCTACTGCGCGCCGTGCCCTTGTTGCAGGGCAGCCGCGCTCAGCAAGA
CTACCGGGAAGCGCTTGAATTGGTGGAATACCTGCTGGAAAACGACGAACACCATCCGCTGATTGACATGCTGGCAAAAA
AGATCGCCGAATATGAAGACAACAGCGCCGAATTTACCGCATTCAACCAGCGTATCGCCCAGTTACCACAGGGGGTAGCC
GCGCTTCGCGTCCTTATGGAACAGCACCAACTCAGCCAAAGTGATTTTGAACATGAGATCGGTAAAAAGTCGTTGGTTAA
CCAAATCCTGAACGGTAAAAGATCGCTGACCATTCCGCACATTATGGCGCTGGCCAAGCGTTTCAATCTGCCCCCGGCCC
TGTTCCTGCCGGAAGCGGATTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References