Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3844363..3844982 Replicon chromosome
Accession NZ_CP055119
Organism Klebsiella pneumoniae strain SWHIN_100

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HUZ80_RS18800 Protein ID WP_002892050.1
Coordinates 3844764..3844982 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag HUZ80_RS18795 Protein ID WP_002892066.1
Coordinates 3844363..3844737 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ80_RS18785 (3839515) 3839515..3840708 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HUZ80_RS18790 (3840731) 3840731..3843877 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
HUZ80_RS18795 (3844363) 3844363..3844737 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
HUZ80_RS18800 (3844764) 3844764..3844982 + 219 WP_002892050.1 HHA domain-containing protein Toxin
HUZ80_RS18805 (3845141) 3845141..3845707 + 567 WP_002892030.1 maltose O-acetyltransferase -
HUZ80_RS18810 (3845679) 3845679..3845819 - 141 WP_004147370.1 hypothetical protein -
HUZ80_RS18815 (3845840) 3845840..3846310 + 471 WP_002892026.1 YlaC family protein -
HUZ80_RS18820 (3846285) 3846285..3847736 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
HUZ80_RS18825 (3847837) 3847837..3848535 + 699 WP_004177238.1 GNAT family protein -
HUZ80_RS18830 (3848532) 3848532..3848672 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
HUZ80_RS18835 (3848672) 3848672..3848935 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T161622 WP_002892050.1 NZ_CP055119:3844764-3844982 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T161622 NZ_CP071232:c4454587-4454333 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGAACAAACGACACCAAAAAACGCTGTCAGATGTGTTTGCCCGGCCTGTCAACGGTTCTATAAAGTGGTCTGATATTGA
GGCGCTTTTTACCGCATTAGGGGCGGAGATTCACGAAAGGGAAGGTTCTAGGATCGCGGTGCTGTTGAAAGGTGAAAAAA
GAGTCTTTCACCGGCCACACCCCAGACCTACCACTGACAAGGGGGCGGTTAACTCCATTCGGATCTGGTTGGATAGCTTA
GGAATAAAACCATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT161622 WP_002892066.1 NZ_CP055119:3844363-3844737 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT161622 NZ_CP071232:c4454333-4453992 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGAATAACACACTGAAAATTGACGGCCATACGGCCGTCATCAACTTCGACCCTGAAATTGAAATGTTCCGGGGCGAGTT
TGTCGGGCTGAATGGCGGCGCCGACTTCTACGCCTACAGCGTGGACGAGCTGAAGAAAGAAGGCGCGATCTCACTCGCGG
TCTTTCTCGATGAGTGCCATAAAGACGGCATCGAGCCTTACAAGTCGTACAGCGGCAAAGTAACCACCCGCCTGTCGCCG
GAACGCCATCAGGCGTTAGCCATTGCCGCACAGGCCACCGGGCAGTCGATTAATGAACTGCTGAATGAAGGTGTTGATCT
GGTTATCGAAAAGCATTCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References