Detailed information of TA system TA161607

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 85975..86600
• Replicon: plasmid unnamed1
• Accession: NZ_CP055114
• Organism: Shigella flexneri strain SWHIN_101

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HUZ81_RS24450
• Protein ID: WP_000911313.1
• Coordinates: 86202..86600 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: HUZ81_RS24445
• Protein ID: WP_000450520.1
• Coordinates: 85975..86202 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ81_RS24445 (85975)	85975..86202	+	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin
HUZ81_RS24450 (86202)	86202..86600	+	399	WP_000911313.1	type II toxin-antitoxin system VapC family toxin	Toxin
HUZ81_RS24455 (86609)	86609..88762	-	2154	WP_000009324.1	type IV conjugative transfer system coupling protein TraD	-
HUZ81_RS24460 (89015)	89015..89746	-	732	WP_023565183.1	conjugal transfer complement resistance protein TraT	-
HUZ81_RS24465 (89778)	89778..90275	-	498	WP_000605857.1	entry exclusion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	oqxA / oqxB / sul2 / aph(3'')-Ib / aph(6)-Id / aph(3')-Ia / sitABCD	iroN / iroE / iroD / iroC / iroB / iutA / iucD / iucC / iucB / iucA	1..131712	131712

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14901.15 Da
• Isoelectric Point: 7.8605

>T161607 WP_000911313.1 NZ_CP055114:86202-86600 [Shigella flexneri]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T161607 NZ_CP071230:c1108032-1107829 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGACTAAAACCGACTATCTGATGCGTTTACGTAAATGCACCACGATTGATACTCTCGAACGTGTTATTGAGAAAAATAA
GTACGAGCTTTCCGATGATGAACTGGAGCTGTTTTACTCAGCGGCCGATCACCGTCTGGCAGAGCTCACCATGAACAAAC
TGTATGACAAAATCCCCACCTCCGTTTGGAAATATGTCAGATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT161607 WP_000450520.1 NZ_CP055114:85975-86202 [Shigella flexneri]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT161607 NZ_CP071230:c1108450-1108082 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGGATGAGTATTCGCCTAAGCGGCACGACATTGCTCAGCTCAAATTCTTGTGCGAAAATTTGTATGATGAAGGTATCGC
TACCCTGGGAGACAGTCATCATGGGTGGGTCAACGATCCGACGTCCTCCGTCAACCTGCAGCTTAATGAGTTGATCGAAC
ATATCGCTTCGTTTGTGATGAGTTATAAAATTAAATACATGGACGAATCTGACCTGTCGGAGTTAGTCGAAGAATATCTC
GACGATACCTATACGCTGTTCAGCAGCTATGGTATCAATGATTCCGACCTGCGCCGCTGGCAAAAAACCAAGGCGAGATT
ATTCAGAATGTTCTCAGGAGAGGACATCTGTACGACAATGAAAACTTAG

Structures

Antitoxin

• AlphaFold DB: V0VCB2