Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4080285..4080939 Replicon chromosome
Accession NZ_CP055113
Organism Shigella flexneri strain SWHIN_101

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag HUZ81_RS19785 Protein ID WP_000244772.1
Coordinates 4080532..4080939 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HUZ81_RS19780 Protein ID WP_000354046.1
Coordinates 4080285..4080551 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ81_RS19755 (4075573) 4075573..4076316 + 744 WP_000951964.1 SDR family oxidoreductase -
HUZ81_RS19760 (4076373) 4076373..4077806 - 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
HUZ81_RS19765 (4077851) 4077851..4078162 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
HUZ81_RS19770 (4078326) 4078326..4078985 + 660 WP_000250274.1 hemolysin III family protein -
HUZ81_RS19775 (4079062) 4079062..4080042 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
HUZ81_RS19780 (4080285) 4080285..4080551 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HUZ81_RS19785 (4080532) 4080532..4080939 + 408 WP_000244772.1 protein YgfX Toxin
HUZ81_RS19790 (4080979) 4080979..4081500 - 522 WP_001055874.1 flavodoxin FldB -
HUZ81_RS19795 (4081612) 4081612..4082508 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HUZ81_RS19800 (4082533) 4082533..4083243 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HUZ81_RS19805 (4083249) 4083249..4084982 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T161600 WP_000244772.1 NZ_CP055113:4080532-4080939 [Shigella flexneri]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T161600 NZ_CP071228:2039681-2039777 [Serratia marcescens subsp. marcescens ATCC 13880]
AACAAGCCCTGCATTAAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTAGACCGAATATAGGAATC
GTATTCGGTCTTTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT161600 WP_000354046.1 NZ_CP055113:4080285-4080551 [Shigella flexneri]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT161600 NZ_CP071228:c2039777-2039637 [Serratia marcescens subsp. marcescens ATCC 13880]
AAAAAAAGACCGAATACGATTCCTATATTCGGTCTAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCAT
TTAATGCAGGGCTTGTTCAGCCGTGCACTTTAAGAGTAGCCTACCGCGCCAGTTTTGCCAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References