Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3443504..3444135 Replicon chromosome
Accession NZ_CP055113
Organism Shigella flexneri strain SWHIN_101

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag HUZ81_RS16570 Protein ID WP_001259388.1
Coordinates 3443504..3443779 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag HUZ81_RS16575 Protein ID WP_000593555.1
Coordinates 3443776..3444135 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ81_RS16555 (3438508) 3438508..3439575 + 1068 WP_000361485.1 HlyD family secretion protein -
HUZ81_RS16560 (3439572) 3439572..3442307 + 2736 WP_000149125.1 ribosome-associated ATPase/putative transporter RbbA -
HUZ81_RS16565 (3442307) 3442307..3443431 + 1125 WP_001314210.1 ABC transporter permease -
HUZ81_RS16570 (3443504) 3443504..3443779 + 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
HUZ81_RS16575 (3443776) 3443776..3444135 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
HUZ81_RS16580 (3444255) 3444255..3444656 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
HUZ81_RS16585 (3444662) 3444662..3445468 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
HUZ81_RS16590 (3445465) 3445465..3446229 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
HUZ81_RS16595 (3446229) 3446229..3447062 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
HUZ81_RS16600 (3447059) 3447059..3448003 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T161597 WP_001259388.1 NZ_CP055113:3443504-3443779 [Shigella flexneri]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T161597 NZ_CP071228:55329-55604 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGGAATTAAAATGGACGAGTAAGGCGCTATCGGATTTGGCGCGTTTGTTTGAGTTTCTTGCCTTGGCTAACCGCACTGC
GGCAGCGAATTCGGTGAAGGCCTTGGTCGCCGCGCCTAAAGCGTTGCTCGATAATCCTCGCCTGGGTGAAAGACTGGATG
AGTTTTTACCGCAGGAAGTGAGGCGTATTCTGGTCGGCCGTTATGAGATACGTTACCAGGTGCAGCCGGCAACGATTTAT
GTTCTGCGTATTTGGCATACCCGCGAAGAGCGATAG

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT161597 WP_000593555.1 NZ_CP055113:3443776-3444135 [Shigella flexneri]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT161597 NZ_CP071228:55069-55329 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGAATACGAAAGTCATCACCGCTCATGTGCCGCTGCCTTTGGCTGAAAAGGTGGATGAAATGGCCGCCAAACTTGAACG
TTCACGGGGATGGATAGTGAAGCAAGCCCTCGCCGCCTGGCTTGAGCAAGAGGAAGAACGCCGTCGATTAACGCTGGCGG
CGCTGGCCGATGTCGAACAGCAAAACGTTATCGATCATGAGTCGGTAGAAGCCTGGGCTGCCAGCCTGGACAGTGACGAG
CCGCTGCCGGTACCGCGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References