Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 2523109..2523704 Replicon chromosome
Accession NZ_CP055113
Organism Shigella flexneri strain SWHIN_101

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag HUZ81_RS12245 Protein ID WP_000239579.1
Coordinates 2523109..2523459 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag HUZ81_RS12250 Protein ID WP_001223208.1
Coordinates 2523453..2523704 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ81_RS12225 (2518385) 2518385..2519407 - 1023 WP_001296689.1 ABC transporter permease -
HUZ81_RS12230 (2519421) 2519421..2520923 - 1503 WP_000205794.1 sugar ABC transporter ATP-binding protein -
HUZ81_RS12235 (2521233) 2521233..2522189 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
HUZ81_RS12240 (2522499) 2522499..2523029 + 531 WP_000055072.1 inorganic diphosphatase -
HUZ81_RS12245 (2523109) 2523109..2523459 - 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
HUZ81_RS12250 (2523453) 2523453..2523704 - 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
HUZ81_RS12255 (2523916) 2523916..2524257 - 342 WP_001219160.1 gamma-glutamylcyclotransferase -
HUZ81_RS12260 (2524260) 2524260..2528039 - 3780 WP_000060895.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T161593 WP_000239579.1 NZ_CP055113:c2523459-2523109 [Shigella flexneri]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T161593 NZ_CP071226:c3786732-3786343 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGTACATGTTTGATACCAACACCGTCAGCCACTTGTTTCGGCAGCATCCGCAGGTGCTTAACGTCATGGAAAAGCTCCC
GCCTTCTGCCGTATGTATTTCCAGCGTCACGGAGGCGGAACTGCTTTACGGTGTGGCTAAACGGCGGAACAAGGCGTTGC
AAAGCATGGTCGAGGCCTTTCTTGCTGCAGTGACGGTCTATGCATGGGATAGCGAGGCCGCCCGTTGCTACGGTGAAATG
CGGGCAACCATGGAGAGGAAGGGCAAAATCATGGGCGCGATCGATCAACTGATCGCCGCTCACGCGCAGAGCCGTGGCGC
GACGCTGGTGACCAACGATCGCGCATTCGCCATGGTGCCCGGGTTGGCGGTTGAAGACTGGACGCGCTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT161593 WP_001223208.1 NZ_CP055113:c2523704-2523453 [Shigella flexneri]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT161593 NZ_CP071226:c3786999-3786736 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGGAAAGAATCGCAAAGCTGTTTAAAAACGGCAGAAATCAGGCGGTGAGGTTACCGGTGGAATTTGAATTTGATACGGA
TCGTGTCTATATCCGTCGCGATCGGGAGGGCAACGTGATTTTATCCAAATATCCGGCAAAGCCGGACAGCTGGGCACCGC
TGCTGAGCATTATCGCCCAAACGCCGGTGCCGCAAGAATTTCTTGGCGCGGAAGATCGTCAGCAAGGGATGGCAGACCGC
GATCCGTTCTCAGAGGAAGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References