Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1773307..1773925 Replicon chromosome
Accession NZ_CP055113
Organism Shigella flexneri strain SWHIN_101

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HUZ81_RS08630 Protein ID WP_001291435.1
Coordinates 1773707..1773925 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HUZ81_RS08625 Protein ID WP_000344800.1
Coordinates 1773307..1773681 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ81_RS08615 (1768396) 1768396..1769589 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HUZ81_RS08620 (1769612) 1769612..1772761 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
HUZ81_RS08625 (1773307) 1773307..1773681 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HUZ81_RS08630 (1773707) 1773707..1773925 + 219 WP_001291435.1 HHA domain-containing protein Toxin
HUZ81_RS08635 (1774096) 1774096..1774647 + 552 WP_000102564.1 maltose O-acetyltransferase -
HUZ81_RS08640 (1774763) 1774763..1775233 + 471 WP_000136192.1 YlaC family protein -
HUZ81_RS08645 (1775397) 1775397..1776947 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HUZ81_RS08650 (1776989) 1776989..1777342 - 354 WP_000878141.1 DUF1428 family protein -
HUZ81_RS08660 (1777721) 1777721..1778032 + 312 WP_000409911.1 MGMT family protein -
HUZ81_RS08665 (1778063) 1778063..1778635 - 573 WP_000779826.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T161590 WP_001291435.1 NZ_CP055113:1773707-1773925 [Shigella flexneri]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T161590 NZ_CP071226:1601799-1602107 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGCAATTTACCGTATATGACAATACGTATCCGGCATCAGCCTATCCCTACTTACTGGACGTTCAAAGCGATCTTATTGA
TGTCCTCTCGACCCGGTTAATGATCCCTCTTTATGCTTTGGATAATGTCAGGGTTAAAATTTCTGCACGTTTATGCCCGG
AGATTGAGGTCAACGGCGAAAAGTTTCTTGTTATGACGCATGAGATGGCTGCCGTACGTATTTCGCAGATTGGGAAGGCC
GTTGGTAATGTCAATGAGCACAGGAATCAAATTAAAGCCGCCATCGATTTTCTGATTGATGGTTTTTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT161590 WP_000344800.1 NZ_CP055113:1773307-1773681 [Shigella flexneri]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT161590 NZ_CP071226:1601560-1601796 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGGCAAATAACAATGTGACGAAAAAGACAGTCAACGTAACCATCGATCGCGATCTTTTCCAGCGCGCAAAATCCCTGGG
CGTCAATGTGTCATCTGTATTGACGGACGCATTGAGCGTCCGTGTCAGGGACATTGAGATTCAACAGTGGCGCGAGCAAA
ATCGTGCGGCGTTAGAAGAACTTAATCGTATCACTGAAGAAAATGGTTTGTTGTCTGATGAATATCGGACATTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References