Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 771271..771909 Replicon chromosome
Accession NZ_CP055113
Organism Shigella flexneri strain SWHIN_101

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag HUZ81_RS03785 Protein ID WP_000813794.1
Coordinates 771733..771909 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag HUZ81_RS03780 Protein ID WP_001270286.1
Coordinates 771271..771687 (-) Length 139 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ81_RS03760 (766423) 766423..767364 - 942 WP_001251315.1 ABC transporter permease -
HUZ81_RS03765 (767365) 767365..768378 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
HUZ81_RS03770 (768396) 768396..769541 - 1146 WP_000047456.1 ABC transporter substrate-binding protein -
HUZ81_RS03775 (769786) 769786..771192 - 1407 WP_000760592.1 PLP-dependent aminotransferase family protein -
HUZ81_RS03780 (771271) 771271..771687 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
HUZ81_RS03785 (771733) 771733..771909 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
HUZ81_RS03790 (772131) 772131..772361 + 231 WP_000494244.1 YncJ family protein -
HUZ81_RS03795 (772453) 772453..774414 - 1962 WP_001593323.1 23S rRNA 5-hydroxycytidine C2501 synthase -
HUZ81_RS03800 (774487) 774487..775023 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
HUZ81_RS03805 (775115) 775115..776290 + 1176 WP_001236215.1 BenE family transporter YdcO -
HUZ81_RS03810 (776330) 776330..776644 - 315 Protein_748 transposase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 767365..787824 20459


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(5-57)

Antitoxin

(91-134)


Sequences


Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T161589 WP_000813794.1 NZ_CP055113:c771909-771733 [Shigella flexneri]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T161589 NZ_CP071226:c1108033-1107830 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGACTAAAACCGACTATCTGATGCGTTTACGTAAATGCACCACGATTGATACTCTCGAACGTGTTATTGAGAAAAATAA
GTACGAGCTTTCCGATGATGAACTGGAGCTGTTTTACTCAGCGGCCGATCACCGTCTGGCAGAGCTCACCATGAACAAAC
TGTATGACAAAATCCCCACCTCCGTTTGGAAATATGTCAGATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT161589 WP_001270286.1 NZ_CP055113:c771687-771271 [Shigella flexneri]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT161589 NZ_CP071226:c1108451-1108083 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGGATGAGTATTCGCCTAAGCGGCACGACATTGCTCAGCTCAAATTCTTGTGCGAAAATTTGTATGATGAAGGTATCGC
TACCCTGGGAGACAGTCATCATGGGTGGGTCAACGATCCGACGTCCTCCGTCAACCTGCAGCTTAATGAGTTGATCGAAC
ATATCGCTTCGTTTGTGATGAGTTATAAAATTAAATACATGGACGAATCTGACCTGTCGGAGTTAGTCGAAGAATATCTC
GACGATACCTATACGCTGTTCAGCAGCTATGGTATCAATGATTCCGACCTGCGCCGCTGGCAAAAAACCAAGGCGAGATT
ATTCAGAATGTTCTCAGGAGAGGACATCTGTACGACAATGAAAACTTAG

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 6HPB


Antitoxin

Source ID Structure

References