Detailed information of TA system TA161574

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 1981..2540
• Replicon: plasmid unnamed2
• Accession: NZ_CP055111
• Organism: Shigella flexneri strain SWHIN_104

Toxin (Protein)

• Gene name: relE
• Uniprot ID: U9YB49
• Locus tag: HUZ82_RS24160
• Protein ID: WP_000421259.1
• Coordinates: 2265..2540 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y625
• Locus tag: HUZ82_RS24155
• Protein ID: WP_001178087.1
• Coordinates: 1981..2265 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ82_RS24150 (46)	46..1077	+	1032	WP_000907869.1	plasmid replication initiator RepA	-
HUZ82_RS24155 (1981)	1981..2265	+	285	WP_001178087.1	ribbon-helix-helix domain-containing protein	Antitoxin
HUZ82_RS24160 (2265)	2265..2540	+	276	WP_000421259.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUZ82_RS24165 (2646)	2646..2926	+	281	Protein_3	hypothetical protein	-
HUZ82_RS24170 (3118)	3118..3384	-	267	WP_021513970.1	hypothetical protein	-
HUZ82_RS24175 (3384)	3384..3878	-	495	WP_015058884.1	hypothetical protein	-
HUZ82_RS24180 (3934)	3934..4536	-	603	WP_000521606.1	ProQ/FINO family protein	-
HUZ82_RS24185 (4712)	4712..5764	-	1053	WP_000655519.1	hypothetical protein	-
HUZ82_RS24190 (5977)	5977..6567	-	591	WP_001401589.1	hypothetical protein	-
HUZ82_RS24195 (6567)	6567..6821	-	255	WP_000343106.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..84883	84883

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10736.45 Da
• Isoelectric Point: 9.9324

>T161574 WP_000421259.1 NZ_CP055111:2265-2540 [Shigella flexneri]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T161574 NZ_CP071222:c3109566-3109168 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGTTCAGTCATATGCTGGACACCAACATTGTTATCTATGTCATCAAGCGCCGGCCGCTGGAAGTGCTGGAGGCATTCAA
TCGCTATGCAGGTAAAATGGTCATTTCGTCGATCACTTATGGCGAATTGGTTCACGGGGTGGAGAAAAGCGCTCGTCCAG
CGGTGAACGCCAGAGTCGTTGAGGATTTTGTCTCCCGATTGGACATTCTCGATTACAGCGCCAAAGCGGCATCGCACTAC
GGCAATATCCGGGCTGCGCTGGAACGACAGGGAACGCCTATTGGCGTAAACGATCTACATATTGCCGGGCATGCGCGCAG
CGAAGGATTGATCCTGGTGACCAATAATCGACGCGAATTTGAGCGCGTTGAGGGTTTGCGGCTGGAAAATTGGTTGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10488.92 Da
• Isoelectric Point: 5.6558

>AT161574 WP_001178087.1 NZ_CP055111:1981-2265 [Shigella flexneri]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT161574 NZ_CP071222:c3109799-3109566 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGACGTTAGGTTCTGTCTTTACTAATAATCGGACACAGGCTGTACGCCTGCCTGCAGAGGCGCGTTTCCCTGATACCGT
TTCTAAAGTGACCGTTCGCGTAGTGGGTAAAGAGCGCATTCTTGCCCCCCTGGAGAATACCTGGGACAGTTTCTTCTGTG
CGGAAAATGAGGTGAGCGATGATTTTCTCAATGAACGTGCCGGACAGCACCAGCGCGAGCGGGAAGCGTTTTGA

Structures

Toxin

• AlphaFold DB: U9YB49

Antitoxin

• AlphaFold DB: U9Y625