Detailed information of TA system TA161570

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 122456..123081
• Replicon: plasmid unnamed1
• Accession: NZ_CP055110
• Organism: Shigella flexneri strain SWHIN_104

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HUZ82_RS23800
• Protein ID: WP_000911313.1
• Coordinates: 122683..123081 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: HUZ82_RS23795
• Protein ID: WP_000450520.1
• Coordinates: 122456..122683 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ82_RS23795 (122456)	122456..122683	+	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin
HUZ82_RS23800 (122683)	122683..123081	+	399	WP_000911313.1	type II toxin-antitoxin system VapC family toxin	Toxin
HUZ82_RS23805 (123090)	123090..125243	-	2154	WP_000009324.1	type IV conjugative transfer system coupling protein TraD	-
HUZ82_RS23810 (125496)	125496..126227	-	732	WP_023565183.1	conjugal transfer complement resistance protein TraT	-
HUZ82_RS23815 (126259)	126259..126756	-	498	WP_000605857.1	entry exclusion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / oqxA / oqxB / sul2 / aph(3'')-Ib / aph(6)-Id / floR / blaCTX-M-55 / blaTEM-1B / fosA3 / dfrA14 / dfrA12 / aadA2 / mph(A) / aph(3')-Ia / aac(3)-IId / sitABCD	iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA	1..177080	177080

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14901.15 Da
• Isoelectric Point: 7.8605

>T161570 WP_000911313.1 NZ_CP055110:122683-123081 [Shigella flexneri]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T161570 NZ_CP071222:55329-55604 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGGAATTAAAATGGACGAGTAAGGCGCTATCGGATTTGGCGCGTTTGTTTGAGTTTCTTGCCTTGGCTAACCGCACTGC
GGCAGCGAATTCGGTGAAGGCCTTGGTCGCCGCGCCTAAAGCGTTGCTCGATAATCCTCGCCTGGGTGAAAGACTGGATG
AGTTTTTACCGCAGGAAGTGAGGCGTATTCTGGTCGGCCGTTATGAGATACGTTACCAGGTGCAGCCGGCAACGATTTAT
GTTCTGCGTATTTGGCATACCCGCGAAGAGCGATAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT161570 WP_000450520.1 NZ_CP055110:122456-122683 [Shigella flexneri]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT161570 NZ_CP071222:55069-55329 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGAATACGAAAGTCATCACCGCTCATGTGCCGCTGCCTTTGGCTGAAAAGGTGGATGAAATGGCCGCCAAACTTGAACG
TTCACGGGGATGGATAGTGAAGCAAGCCCTCGCCGCCTGGCTTGAGCAAGAGGAAGAACGCCGTCGATTAACGCTGGCGG
CGCTGGCCGATGTCGAACAGCAAAACGTTATCGATCATGAGTCGGTAGAAGCCTGGGCTGCCAGCCTGGACAGTGACGAG
CCGCTGCCGGTACCGCGCTGA

Structures

Antitoxin

• AlphaFold DB: V0VCB2