Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4314055..4314552 Replicon chromosome
Accession NZ_CP055109
Organism Shigella flexneri strain SWHIN_104

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HUZ82_RS20940 Protein ID WP_001173343.1
Coordinates 4314055..4314228 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag HUZ82_RS20945 Protein ID WP_000398619.1
Coordinates 4314256..4314552 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ82_RS20930 (4310725) 4310725..4312182 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
HUZ82_RS20935 (4312419) 4312419..4313936 + 1518 WP_023155344.1 lysine--tRNA ligase -
HUZ82_RS20940 (4314055) 4314055..4314228 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HUZ82_RS20945 (4314256) 4314256..4314552 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HUZ82_RS20950 (4314779) 4314779..4315051 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
HUZ82_RS20955 (4315063) 4315063..4315293 - 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
HUZ82_RS20960 (4315474) 4315474..4317105 + 1632 WP_001216460.1 sensor histidine kinase -
HUZ82_RS20965 (4317102) 4317102..4317821 + 720 WP_000611287.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T161564 WP_001173343.1 NZ_CP055109:c4314228-4314055 [Shigella flexneri]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T161564 NZ_CP071220:2039699-2039795 [Serratia marcescens subsp. marcescens ATCC 13880]
AACAAGCCCTGCATTAAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTAGACCGAATATAGGAATC
GTATTCGGTCTTTTTTT

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT161564 WP_000398619.1 NZ_CP055109:c4314552-4314256 [Shigella flexneri]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT161564 NZ_CP071220:c2039795-2039655 [Serratia marcescens subsp. marcescens ATCC 13880]
AAAAAAAGACCGAATACGATTCCTATATTCGGTCTAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCAT
TTAATGCAGGGCTTGTTCAGCCGTGCACTTTAAGAGTAGCCTACCGCGCCAGTTTTGCCAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References