Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4218121..4218716 Replicon chromosome
Accession NZ_CP055109
Organism Shigella flexneri strain SWHIN_104

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag HUZ82_RS20445 Protein ID WP_000239579.1
Coordinates 4218121..4218471 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag HUZ82_RS20450 Protein ID WP_001223208.1
Coordinates 4218465..4218716 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ82_RS20425 (4213567) 4213567..4214589 - 1023 WP_001296689.1 ABC transporter permease -
HUZ82_RS20430 (4214603) 4214603..4216105 - 1503 WP_000205800.1 sugar ABC transporter ATP-binding protein -
HUZ82_RS20435 (4216245) 4216245..4217201 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
HUZ82_RS20440 (4217511) 4217511..4218041 + 531 WP_000055072.1 inorganic diphosphatase -
HUZ82_RS20445 (4218121) 4218121..4218471 - 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
HUZ82_RS20450 (4218465) 4218465..4218716 - 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
HUZ82_RS20455 (4218929) 4218929..4219270 - 342 WP_001219160.1 gamma-glutamylcyclotransferase -
HUZ82_RS20460 (4219273) 4219273..4223052 - 3780 WP_001402801.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T161563 WP_000239579.1 NZ_CP055109:c4218471-4218121 [Shigella flexneri]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T161563 NZ_CP071220:1601799-1602107 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGCAATTTACCGTATATGACAATACGTATCCGGCATCAGCCTATCCCTACTTACTGGACGTTCAAAGCGATCTTATTGA
TGTCCTCTCGACCCGGTTAATGATCCCTCTTTATGCTTTGGATAATGTCAGGGTTAAAATTTCTGCACGTTTATGCCCGG
AGATTGAGGTCAACGGCGAAAAGTTTCTTGTTATGACGCATGAGATGGCTGCCGTACGTATTTCGCAGATTGGGAAGGCC
GTTGGTAATGTCAATGAGCACAGGAATCAAATTAAAGCCGCCATCGATTTTCTGATTGATGGTTTTTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT161563 WP_001223208.1 NZ_CP055109:c4218716-4218465 [Shigella flexneri]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT161563 NZ_CP071220:1601560-1601796 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGGCAAATAACAATGTGACGAAAAAGACAGTCAACGTAACCATCGATCGCGATCTTTTCCAGCGCGCAAAATCCCTGGG
CGTCAATGTGTCATCTGTATTGACGGACGCATTGAGCGTCCGTGTCAGGGACATTGAGATTCAACAGTGGCGCGAGCAAA
ATCGTGCGGCGTTAGAAGAACTTAATCGTATCACTGAAGAAAATGGTTTGTTGTCTGATGAATATCGGACATTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References