Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3484821..3485439 Replicon chromosome
Accession NZ_CP055109
Organism Shigella flexneri strain SWHIN_104

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HUZ82_RS16820 Protein ID WP_001291435.1
Coordinates 3485221..3485439 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HUZ82_RS16815 Protein ID WP_000344800.1
Coordinates 3484821..3485195 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ82_RS16805 (3479910) 3479910..3481103 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HUZ82_RS16810 (3481126) 3481126..3484275 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
HUZ82_RS16815 (3484821) 3484821..3485195 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HUZ82_RS16820 (3485221) 3485221..3485439 + 219 WP_001291435.1 HHA domain-containing protein Toxin
HUZ82_RS16825 (3485611) 3485611..3486162 + 552 WP_040091271.1 maltose O-acetyltransferase -
HUZ82_RS16830 (3486278) 3486278..3486748 + 471 WP_000136192.1 YlaC family protein -
HUZ82_RS16835 (3486912) 3486912..3488462 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HUZ82_RS16840 (3488504) 3488504..3488857 - 354 WP_000878141.1 DUF1428 family protein -
HUZ82_RS16850 (3489236) 3489236..3489547 + 312 WP_000409911.1 MGMT family protein -
HUZ82_RS16855 (3489578) 3489578..3490150 - 573 WP_000779826.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T161561 WP_001291435.1 NZ_CP055109:3485221-3485439 [Shigella flexneri]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T161561 NZ_CP071220:55329-55604 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGGAATTAAAATGGACGAGTAAGGCGCTATCGGATTTGGCGCGTTTGTTTGAGTTTCTTGCCTTGGCTAACCGCACTGC
GGCAGCGAATTCGGTGAAGGCCTTGGTCGCCGCGCCTAAAGCGTTGCTCGATAATCCTCGCCTGGGTGAAAGACTGGATG
AGTTTTTACCGCAGGAAGTGAGGCGTATTCTGGTCGGCCGTTATGAGATACGTTACCAGGTGCAGCCGGCAACGATTTAT
GTTCTGCGTATTTGGCATACCCGCGAAGAGCGATAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT161561 WP_000344800.1 NZ_CP055109:3484821-3485195 [Shigella flexneri]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT161561 NZ_CP071220:55069-55329 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGAATACGAAAGTCATCACCGCTCATGTGCCGCTGCCTTTGGCTGAAAAGGTGGATGAAATGGCCGCCAAACTTGAACG
TTCACGGGGATGGATAGTGAAGCAAGCCCTCGCCGCCTGGCTTGAGCAAGAGGAAGAACGCCGTCGATTAACGCTGGCGG
CGCTGGCCGATGTCGAACAGCAAAACGTTATCGATCATGAGTCGGTAGAAGCCTGGGCTGCCAGCCTGGACAGTGACGAG
CCGCTGCCGGTACCGCGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References