Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 856142..856796 Replicon chromosome
Accession NZ_CP055109
Organism Shigella flexneri strain SWHIN_104

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag HUZ82_RS04205 Protein ID WP_000244781.1
Coordinates 856389..856796 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HUZ82_RS04200 Protein ID WP_000354046.1
Coordinates 856142..856408 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ82_RS04175 (851311) 851311..852054 + 744 WP_000951961.1 SDR family oxidoreductase -
HUZ82_RS04180 (852111) 852111..853544 - 1434 WP_032283547.1 6-phospho-beta-glucosidase BglA -
HUZ82_RS04185 (853589) 853589..853900 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
HUZ82_RS04190 (854064) 854064..854723 + 660 WP_000250274.1 hemolysin III family protein -
HUZ82_RS04195 (854919) 854919..855899 - 981 WP_000886095.1 tRNA-modifying protein YgfZ -
HUZ82_RS04200 (856142) 856142..856408 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HUZ82_RS04205 (856389) 856389..856796 + 408 WP_000244781.1 protein YgfX Toxin
HUZ82_RS04210 (856836) 856836..857357 - 522 WP_001055874.1 flavodoxin FldB -
HUZ82_RS04215 (857469) 857469..858365 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HUZ82_RS04220 (858390) 858390..859100 + 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HUZ82_RS04225 (859106) 859106..860839 + 1734 WP_000813223.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T161548 WP_000244781.1 NZ_CP055109:856389-856796 [Shigella flexneri]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T161548 NZ_CP071216:c3787969-3787580 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGTACATGTTTGATACCAACACCGTCAGCCACTTGTTTCGGCAGCATCCGCAGGTGCTTAACGTCATGGAAAAGCTCCC
GCCTTCTGCCGTATGTATTTCCAGCGTCACGGAGGCGGAACTGCTTTACGGTGTGGCTAAACGGCGGAACAAGGCGTTGC
AAAGCATGGTCGAGGCCTTTCTTGCTGCAGTGACGGTCTATGCATGGGATAGCGAGGCCGCCCGTTGCTACGGTGAAATG
CGGGCAACCATGGAGAGGAAGGGCAAAATCATGGGCGCGATCGATCAACTGATCGCCGCTCACGCGCAGAGCCGTGGCGC
GACGCTGGTGACCAACGATCGCGCATTCGCCATGGTGCCCGGGTTGGCGGTTGAAGACTGGACGCGCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT161548 WP_000354046.1 NZ_CP055109:856142-856408 [Shigella flexneri]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT161548 NZ_CP071216:c3788236-3787973 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGGAAAGAATCGCAAAGCTGTTTAAAAACGGCAGAAATCAGGCGGTGAGGTTACCGGTGGAATTTGAATTTGATACGGA
TCGTGTCTATATCCGTCGCGATCGGGAGGGCAACGTGATTTTATCCAAATATCCGGCAAAGCCGGACAGCTGGGCACCGC
TGCTGAGCATTATCGCCCAAACGCCGGTGCCGCAAGAATTTCTTGGCGCGGAAGATCGTCAGCAAGGGATGGCAGACCGC
GATCCGTTCTCAGAGGAAGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References